Main description:

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

Contents:

1. A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies

Moshe Bar-Joseph

2. A Brief Historical Account of the Family Closteroviridae

Giovanni P. Martelli

3. Phenotyping Biological Properties of CTV Isolates

Marcella Russo and Antonino F. Catara

4. CTV Vectors and Interactions with the Virus and Host Plants

Raymond Yokomi

5. Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues

Mariano Cambra, Eduardo Vidal, Carmen Martinez, and Edson Bertolini

6. Detection of Citrus Tristeza Virus and Co-Infecting Viroids

Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, and Francesco Di Serio

7. Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP

Elisavet K. Chatzivassiliou and Grazia Licciardello

8. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus

Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, and Raymond Yokomi

9. Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device

Giuseppe Scuderi, Antonino Catara, and Grazia Licciardello

10. Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay

Dilip Kumar Ghosh, Ashish Warghane, and Kajal Kumar Biswas

11. Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome

Munir Mawassi, Sabrina Haviv, and Ludmila Meslenin

12. Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus

Ana Belen Ruiz-Garcia, Rachelle Bester, Antonio Olmos, and Hans Jacob Maree

13. Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology

David A. Read and Gerhard Pietersen

14. Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction

Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Pena, Luis Navarro, Pedro Moreno, Francesco Di Serio, and Ricardo Flores

15. Proteomic Response of Host Plants to Citrus Tristeza Virus

Milena Santos Doria and Carlos Priminho Pirovani

16. Gene Expression in Citrus Plant Cells Using Helios (R) Gene Gun System for Particle Bombardment

Yosvanis Acanda, Chunxia Wang, and Amit Levy

17. Methods for Producing Transgenic Plants Resistant to CTV

Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Pena