Main description:

This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.

Contents:

1. Bone Imaging: Osteoclast and Osteoblast Dynamics

Junichi Kikuta and Masaru Ishii

2. Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability

Yookyung Jung, Joel A. Spencer, Anthony P. Raphael, Juwell W. Wu, Clemens Alt, Judith R. Runnels, Briaira Geiger, and Charles P. Lin

3. Bone Imaging: Platelet Formation Dynamics

Asuka Sakata and Satoshi Nishimura

4. Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices

Tomoya Katakai

5. Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches

Akira Takeda, Masayuki Miyasaka, and Eiji Umemoto

6. Imaging the Lymph Node Stroma

Clement Ghigo, Rebecca Gentek, and Marc Bajenoff

7. Intravital Imaging of B Cell Responses in Lymph Nodes

Stefano Sammicheli, Mirela Kuka, and Matteo Iannacone

8. Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response

Gyohei Egawa, Tetsuya Honda, and Kenji Kabashima

9. Imaging of Inflammatory Responses in the Mouse Ear Skin

Jackson Liang Yao Li, Chi Ching Goh, and Lai Guan Ng

10. In Vivo Imaging of Immune Cells in Peyer's Patches

Andrea Reboldi

11. Intravital Imaging of T Cells Within the Spinal Cord

Naoto Kawakami

12. Kidney Imaging: Intravital Microscopy

Takashi Hato, Seth Winfree, and Pierre C. Dagher

13. Intravital Imaging of Liver Cell Dynamics

Sayaka Matsumoto, Junichi Kikuta, and Masaru Ishii

14. Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy

Ryohei Matsuura, Shigeru Miyagawa, Junichi Kikuta, Masaru Ishii, and Yoshiki Sawa

15. Imaging Window Device for Subcutaneous Implantation Tumor

Wataru Ikeda, Ken Sasai, and Tsuyoshi Akagi

16. New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR

Michio Tomura