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Main description:
This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.
Contents:
Part I: Introduction
1. Overview of Microarray Technology
Paul C. H. Li
Part II: Advances on Techniques in Microarray Technology
2. Surface Functionalization for Immobilization of Probes on Microarrays
C. Desmet and C.A. Marquette
3. The "clickable" Method for Oligonucleotide Immobilization onto Azide-Functionalized Microarrays
Tomasz Ratajczak, Barbara Uszczynska, Emilia Frydrych-Tomczak, and Marcin K. Chmielewski
4. Microarray Developed on Plastic Substrates
Maria-Jose Banuls; Sergi B. Morais, Luis A. Tortajada-Genaro, and Angel Maquieira
5. Detection and Quantification of Micrornas by Ligase-Assisted Sandwich Hybridization on a Microarray
Ryo Iizuka, Taro Ueno, and Takashi Funatsu
6. Probe Design Strategies for Oligonucleotide Microarrays
Nicolas Parisot, Eric Peyretaillade, Eric Dugat-Bony, Jeremie Denonfoux, Antoine Mahul, and Pierre Peyret
PART III: Applications of Microarray Technology to Nucleic Acid Assays
7. Analyzing Illumina Gene Expression Microarray Data Obtained from Human Whole Blood Cell and Blood Monocyte Samples
Alexander Teumer, Claudia Schurmann, Arne Schillert, Katharina Schramm, Andreas Ziegler, and Holger Prokisch
8. Microarray Technology and its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers
Hirotaka Konishi, Daisuke Ichikawa, Tomohiro Arita, and Eigo Otsuji
9. Application of DNA Microarray to Clinical Diagnostics
Ankita Patel and Sau W. Cheung
10. High-throughput DNA Array for SNP Detection of KRAS Gene using a Centrifugal Microfluidic Device
Abootaleb Sedighi and Paul C. H. Li
11. Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies
Annalisa Lonetti, Maria Chiara Fontana, Giovanni Martinelli, and Ilaria Iacobucci
12. DNA Microarray-Based Diagnostics
Mahsa Gharibi Marzancola, Abootaleb Sedighi, and Paul C. H. Li
Part IV: Applications of Microarray Technology to Other Biological Assays
13. High-throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) On Phosphopeptide Microarrays
Liqian Gao, Su Seong Lee, Jun Chen, Hongyan Sun, Yuliang Zhao, Zhifang Chai, and Yi Hu
14. Nanotechnology in the Fabrication of Protein Microarrays
Manuel Fuentes, Paula Diez, and Juan Casado-Vela
15. Epitope Mapping using Peptide Microarray in Autoantibody Profiling
Sebastian Henkel, Robert Wellhausen, Dirk Woitalla, Katrin Marcus, and Caroline May
16. Preparation of Glycan Arrays using Pyridylaminated Glycans
Shin-ichi Nakakita and Jun Hirabayashi
17. Competitive Immunoassays Using Antigen Microarrays
Zhaowei Zhang, Weihua Hu, Qi Zhang, Peiwu Li, and Changming Li
18. Parallel Syntheses of Peptides on Teflon-patterned paper arrays (SyntArrays)
Frederique Deiss, Yang Yang, and Ratmir Derda
19. Cell Microarrays for Biomedical Applications
Mario Rothbauer, David Wartmann, Verena Charwat, and Peter Ertl
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2015
Pages: 250
Weight: 7938g
Availability: Available
Subcategories: Genetics
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