Main description:

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Contents:

1. Media culturing methods for selection and biochemical identification of specific Yersinia phenotypes 2. Plague Vertebrate Infection models: Intradermal inoculation

3. Plague Vertebrate Infection models Intranasal inoculation

4. Plague Vertebrate Infection models Aerosol inoculation

5. Plague Vertebrate Infection models Septicemic inoculation

6. Plague Vertebrate Infection models Flea-borne transmission

7. Plague Invertebrate Infection model: Caenorhabditis elegans

8. Plague Invertebrate Infection models: Flea Model

9. Plague Invertebrate Infection models: Drosophila Model

10. Yersiniosis Infection models: Oral gavage

11. Immune cell infection model: Macrophage model

12. Immune cell infection model: Neutrophil model

13. Intravital microscopy of early bubonic plague events

14. Bioluminescence imaging to track bacterial colonization and dissemination

15. Mutagenesis of the Yersinia spp

16. Dual tissue RNAseq

17. Thermosensor RNA regulation

18. The Type III secretion system (T3SS): in vitro

19. The Type III secretion system (T3SS): in vitro

20. In vivo gene expression

21. Metal studies for plague

22. The Yersinia Inflammasome

23. Proteomic Bioforensic detection methods