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MORE ABOUT THIS BOOK
Main description:
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.
Contents:
Table of Contents
Preface
Contributors
Part 1 - Imaging of fixed cells and tissues
1. Immunofluorescent Labeling of Proteins in Cultured Cells with Quantum Dot Secondary Antibody Conjugates
Richard L. Ornberg and Hongjian Liu
2. Immunohistochemical Detection with Quantum Dots
Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio and Kevin A. Roth
3. Quantum Dots for Multicolor Clinical Pathology and Multispectral Imaging
Johbu Itoh and Robert Yoshiyuki Osamura
4. Light and Electron Microscopic Localization of Multiple Proteins using Quantum dots
Thomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone and Mark H. Ellisman
5. Human Metaphase Chromosome FISH using Quantum Dot Conjugates
Joan H.M. Knoll
Part 2 - Imaging of live cells
6. Biotin-Ligand Complexes with Streptavidin Quantum Dots for in vivo Cell Labeling of Membrane Receptors
Diane S. Lidke, Peter Nagy, Thomas M. Jovin and Donna J. Arndt-Jovin
7. Single Quantum Dot Tracking of Membrane Receptors
Cédric Bouzigues, Sabine Lévi, Antoine Triller and Maxime Dahan
8. Optical Monitoring of Single Cells Using Quantum Dots
Jyoti K. Jaiswal and Sanford M. Simon
9. Peptide Mediated Intracellular Delivery of Quantum Dots
B. Christoffer Lagerholm
10. High Content Analysis in Multiple Cell Lines Using Quantum Dots
Paul G. Wylie
11. Measuring Cell Motility Using Quantum Dot Probes
Weiwei Gu, Teresa Pellegrino, Wolfgang J. Parak, Rosanne Boudreau, Mark A. Le Gros, A. Paul Alivisatos and Carolyn A. Larabell
Part 3 - Imaging of live animals
12. Quantum Dots for In-vivo Molecular and Cellular Imaging
Xiaohu Gao, Leland WK Chung and Shuming Nie
13. Sentinel Lymph Node Mapping with Type II Quantum Dots
John V. Frangioni, Sang-WookKim, Shunsuke Ohnishi, Sungjee Kim and Moungi Bawendi
14. Macrophage-mediated Co-localization of Quantum Dots in Experimental Glioma
Osman Muhammad, Alexandra Popescu and Steven A. Toms
Part 4 - Flow cytometry applications
15. Applications of Quantum Dots to Multicolor Flow Cytometry
Pratip K. Chattopadhyay and Mario Roederer
16. Quantum Dots in Flow Cytometry
Barnaby Abrams and Tim Dubrovsky
Part 5 - Biochemical applications
17. Luminescent Biocompatible Quantum Dots: A tool for immunosorbent assay design
Ellen R. Goldman, H. Tetsuo Uyeda, Andrew Hayhurst, and Hedi Mattoussi
18. Fluorescence-based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots
David H. Geho, J. Keith Killian, Animesh Nandi, Johanne Pastor, Prem Gurnani and Kevin P. Rosenblatt.
19. Application of Quantum Dots to Multi-Color Microarray Experiments: Four-Color Genotyping
George Karlin-Neumann, Marina Sedova, Mat Falkowski, Zhiyong Wang, Steven Lin and Maneesh Jain
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2007
Pages: 258
Weight: 622g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry, Genetics
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CUSTOMER REVIEWS
From the reviews:
"...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007
"Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. … in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)