Main description:

This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors.

This is an open access book.

Contents:

1. Optical Manipulation and Recording of Neural Activity with Wavefront Engineering
Ruth R. Sims, Imane Bendifallah, Kris Blanchard, Dimitrii Tanese, Valentina Emiliani, and Eirini Papagiokoumou

2. Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments
Peter Quicke, Carmel L. Howe, and Amanda J. Foust

3. Light-Based Neuronal Circuit Probing in Living Brains at High-Resolution: Constraints and Layouts for Integrating Neuronal Activity Recording and Modulation in Three Dimensions
Mateo Bruzzone, Enrico Chiarello, Andrea Maset, Aram Megighian, Claudia Lodovichi, and Marco dal Maschio

4. High Speed All-Optical Neural Interfaces with 3D Temporally Focused Holography
Ian Anton Oldenburg, Hayley Anne Bounds, and Nicolas C. Pegard

5. An All-Optical Physiology Pipeline towards Highly Specific and Artefact-Free Circuit Mapping
Hendrik Backhaus, Nicolas Ruffini, Anna Wierczeiko, and Albrecht Stroh

6. Spatial and Temporal Considerations of Optogenetic Tools in an All-Optical Single-Beam Experiment
Damaris Holder and Matthias Prigge

7. Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals
Baris N. Ozbay, Gregory L. Futia, Ming Ma, Connor McCullough, Michael D. Young, Diego Restrepo, and Emily A. Gibson

8. Optogenetics and Light Sheet Microscopy
Laura Maddalena, Paolo Pozzi, Nicolo G. Ceffa, Bas van der Hoeven, and Elizabeth C. Carroll

9. Widefield Multiphoton Imaging at Depth with Temporal Focusing
Philip Wijesinghe and Kishan Dholakia

10. High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Guanghan Meng, Qinrong Zhang, and Na Ji

11. Optical and Analytical Methods to Visualize and Manipulate Contrical Ensembles and Behavior
Luis Carrillo-Reid, Weijian Yang, and Rafael Yuste

12. Illuminating Neural Computation using Precision Optogenetics-Controlled Synthetic Perception
Jonathan V. Gill, Gilad M. Lerman, Edmund Chong, Dmitry Rinberg, and Shy Shoham

13. Spectrally-Focused Stimulated Raman Scattering (sf-SRS) Microscopy for Label-Free Investigations of Molecular Mechanisms in Living Organisms
Tamas Vaczi, Laszlo Himics, Matteo Bruzzone, Miklos Veres, and Marco dal Maschio