Main description:

This volume explores the latest techniques used to study the human brain towards understanding Alzheimer's Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural cellular models using primary and human induced pluripotent stem cells to model aspects of the human brain. Part Three details nucleic acid analyses including transcriptomic and somatic genomic changes, and Part Four discusses lipid analyses via mass spectrometry. Lastly, Part Five covers protein analyses, particularly A and Tau. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Alzheimer's Disease: Methods and Protocols is a valuable tool for all researchers who want to expand their knowledge and understanding of this disease and other related dementias.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I Human Brain Preparations

1. Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue
Caitlin S. Latimer, Erica J. Melief, Jeanelle Ariza-Torres, Kim Howard, Amanda R. Keen, Lisa M. Keene, Aimee M. Schantz, Trevor M. Sytsma, Angela M. Wilson, Thomas J. Grabowski, Martin Darvas, Kristen Dams O'Connor, Amber L. Nolan, Brian L. Edlow, Christine L. Mac Donald, and C. Dirk Keene

2. Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue
Carter R. Palmer and Jerold Chun

3. Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era
Lih-Fen Lue, Douglas G. Walker, Suet Theng Beh, and Thomas G. Beach

4. Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images
Marie-Kim St-Pierre, Eva Simoncicova, Micael Carrier, and Marie-Eve Tremblay

5. Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer's Disease
Maria Merlini, Elif G. Sozmen, Keshav S. Subramanian, Alissa L. Nana, William W. Seeley, and Katerina Akassoglou

Part II Human Neural Cellular Models

6. Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer's Disease
Jessica E. Young and Lawrence S.B. Goldstein

7. Modeling Alzheimer's Disease using Human Brain Organoids
Karina Karmirian, Mariana Holubiec, Livia Goto-Silva, Ivan Fernandez Bessone, Gabriela Vitoria, Beatriz Mello, Matias Alloatti, Bart Vanderborght, Tomas L. Falzone, and Stevens Rehen

8. 3D Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology
Tohid Siddiqui, Hilal Celikkaya, Zeynep Tansu Atasavum, Stanislava Popova, Uwe Freudenberg, Carsten Werner, and Caghan Kizil

Part III Nucleic Acid Analyses of Human Brain

9. Computational Approaches to Assess Abnormal Metabolism in Alzheimer's Disease Using Transcriptomics
Hatice Busra Luleci, Dilara Uzuner, Tunahan Cakir, and Madhav Thambisetty

10. FISHing for Chromosome Instability and Aneuploidy in the Alzheimer's Disease Brain
Yuri B. Yurov, Svetlana G. Vorsanova, and Ivan Y. Iourov

11. Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Post-Mortem Human Brain
Diego Perez-Rodriguez, Maria Kalyva, Catherine Santucci, and Christos Proukakis

Part IV Lipids Analyses of Human Brain

12. Mass Spectrometry Analysis of the Human Brain Sphingolipidome
Xin Ying Chua, Ryan Huang, Deron Herr, Mitchell K.P. Lai, Markus R. Wenk, and Federico Torta

13. Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer's Research
Jonatan Martinez-Gardeazabal, Marta Moreno-Rodriguez, Estibaliz Gonzalez de San Roman, Beatriz Abad, Ivan Manuel, and Rafael Rodriguez-Puertas

Part V Protein Analyses of Human Brain

14. The Use of Affinity Purification-Mass Spectrometry to identify Phosphorylates Tau Interactors in Alzheimer's Disease
Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski, and Eleanor Drummond

15. In Vitro Amplification of Pathogenic Tau Seeds from Neurodegenerative Disease Patient Brains
Hong Xu and Virginia M.-Y. Lee

16. A and Tau Prions Causing Alzheimer's Disease
Carlo Condello, Gregory E. Merz, Atsushi Aoyagi, William F. DeGrado, and Stanley B. Prusiner

Subject Index List...