Main description:

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method; another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Contents:

Dedication...Preface...Table of Contents...Contributing Authors...
1. Ultra-Sensitive Quantification of Recombinant Proteins using AAA-MSMathilde Louwagie, Sylvie Kieffer-Jaquinod, and Virginie Brun
2. Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass SpectrometryOlga A. Mirgorodskaya, Roman Koerner, Yuri P. Kozmin, and Peter Roepstorff
3. Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass SpectrometryNatalia V. Gogichaeva and Michail A. Alterman
4. Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen ContentMichelle L. Colgrave, Peter G. Allingham, Kerri Tyrrell, and Alun Jones
5. Analysis of Hydroxyproline in Collagen HydrolysatesTobias Langrock and Ralf Hoffmann
6. Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope Resolved Metabolomics using Ultrahigh Resolution Mass Spectrometry Coupled with Direct InfusionYe Yang, Teresa W.M. Fan, Andrew N. Lane, and Richard M. Higashi
7. Preparative HPLC Separation of Underivatized Amino Acids for Isotopic AnalysisJennifer A. Tripp, Thibaut Deviese, and James S. O. McCullagh
8. Quantitative Analysis of Underivatized Amino Acids by Liquid Chromatography-Tandem Mass SpectrometryJustin Mak, Tina M. Cowan, and Anthony Le
9. Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass SpectrometryMegumi Kato and Akiko Takatsu
10. The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and other Amino AcidsAbdulla A.-B. Badawy
11. Analysis of Tryptophan and Its Metabolites by High-Performance Liquid ChromatographyZhaolai Dai, Shiqiang Sun, Hui Chen, Moyan Liu, Lianhua Zhang, Zhenlong Wu, Ju Li, and Guoyao Wu
12. Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid AnalysisDavid M. Bunk and Mark S. Lowenthal
13. Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MSGiuseppe Giordano, Antonina Gucciardi, Paola Pirillo, and Mauro Naturale
14. Amino Acid Analysis in Physiological Samples by GC-MS with Propylchloroformate Derivatization and iTRAQ-LC-MS/MS Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, Hannelore Kaspar, and Peter J. Oefner
15. Combination of an AccQ Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino AcidsCarolina Salazar, Jenny M. Armenta, Diego F. Cortes, and Vladimir Shulaev
16. Methods for Absolute Quantification of Human Plasma Free Amino Acids by High-Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry using Precolumn DerivatizationAkira Nakayama, Akira Imaizumi, and Hiroo Yoshida
17. Analysis and Enantioseparation of Amino Acids by Liquid ChromatographyPoonam Malik and Ravi Bhushan
18. Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas ChromatographyPetr Simek, Petr Husek and Helena Zahradnickova
19. High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis without Derivatization Yosuke Nakano, Moyu Taniguchi, Yutaka Umakoshi, Daisuke Watai, and Eiichiro Fukusaki
20. A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino AcidsSonja Hess
21. Chiral Analysis of Non-Protein Amino Acids by Capillary ElectrophoresisMaria Castro-Puyana and Maria Luisa Marina
22. Amino Acid Analysis via LC-MS Method after Derivatization with Quaternary PhosphoniumShinsuke Inagaki and Toshimasa Toyo'oka
23. Amino Acid Analysis by Capillary Electrophoresis-Mass SpectrometryAkiyoshi Hirayama, Satsuki Ikeda, Asako Sato, and Tomoyoshi Soga
24. Capillary Electrophoresis of Free Amino Acids in Physiological Fluids without Derivatization Employing Direct or Indirect Absorbance DetectionGordana D. Zunic, Slavica Spasic, and Zorana Jelic-Ivanovic
25. New Advances in Amino Acid Profiling in Biological Samples by Capillary Electrophoresis-Mass SpectrometryMeera Shanmuganathan and Philip Britz-McKibbin
26. Enhanced Separation Capability of Sequential Injection Chromatography for Fluorimetric Determination of Intracellular Dissolved Free Amino Acids in Marine MicroalgaeMarilda Rigobello-Masini and Jorge C. Masini
27. Sensitive and Selective Amino Acid Profiling of Minute Tissue Amounts by HPLC/Electrospray Negative Tandem Mass Spectrometry using 9-Fluorenylmethoxycarbonly (Fmoc-Cl) DerivatizationJoerg Ziegler, Hina Hussain, Reinard H.H. Neubert, and Steffen Abel
28. Wheat Gluten Amino Acid Analysis by High-Performance Anion-Exchange Chromatography with Integrated Pulsed Amperometric DetectionIne Rombouts, Bert Lagrain, Lieve Lamberts, Inge Celus, Kristof Brijs, and Jan A. Delcour
29. Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass SpectrometryMadeleine Dell'mour, Gunda Koellensperger, and Stephan Hann
30. Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS (Improved Method)Bjoern Thiele, Michelle Hupert, Beatrix Santiago-Schubel, Marco Oldiges, and Diana Hofmann
31. Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with On-Line Multiple Detection A. Pappa-Louisi, P. Agrafiotou, and S. Sotiropoulos
32. Non-Marking Collection of Amino Acids from Fingerprints using HydrogelsWard van Helmond, Vincent O'Brien, Robin de Jong, Jan van Esch, Sander Oldenhof, and Marcel de Puit 33. Amino Acid Profiling from Fingerprints using Amide Stationary Phase UPLC-MSWard van Helmond and Marcel de Puit