Main description:

This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.

Contents:

Direct analysis of sedimentation equilibrium distributions reflecting macromolecular interactions.- Alternative strategies for the characterization of associations in multicomponent solutions via measurement of sedimentation equilibrium.- Non-linear curve-fitting methods for data from the XL-A analytical utltracentrifuge.- Rapid molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles.- A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions.- Calculation of hydrodynamic parameters of biopolymers from scattering data using whole-body approaches.- Calculation of hydrodynamic parameters of proteins from crystallographic data using multibody approaches.- Analytical ultracentrifugation with fluorescence detection and biosafety containment and its application to the prion protein.- Dye-labelling as a means to study ternary protein complexes by analytical ultracentrifugation: the band 3/ankyrin/aldolase complex from erythrocyte membranes.- Studies of ligand-mediated conformational changes in enzymes by difference sedimentation velocity in the Optima XL-A ultracentrifuge.- Quaternary structure and interaction parameters of bovine ?-crystallin: influence of isolation conditions.- Investigation of irradiated eye-lens proteins by analytical ultracentrifugation and other techniques.- Sedimentation analysis of SDS and albumin-SDS complexes.- The self-association of basic helix-loop-helix peptides.- Nuclear DNA fractions with grossly different base ratios in the genome of the marine sponge Geodia cydonium.- Sedimentation equilibrium studies of synthetic polyelectrolytes by means of interference optical methods.- Investigation of quantum size colloids using the XL-I ultracentrifuge.- Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge I extended Hermans-Ende equation for the equilibrium density gradient.- Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge. II direct refractometric determination of the equilibrium and non-equilibrium density gradient.- First experiences with the new XL-I AUC: Applications in polymer and colloid science.- Analytical ultracentrifugation as a tool in supramolecular chemistry: a feasibility study using a metal coordination array.- The sedimentation velocity of a gelled polymer.- New contributions of analytical ultracentrifugation to the investigation of dispersions.- Determination of hydrodynamic radius: a comparison of ultracentrifuge methods with dynamic light scattering.