Main description:

This book is a compendium of chapters focusing on the application of CRISPR-Cas 9 in translational research. The initial chapter provides the brief history and basic mechanism of the CRISPR/Cas 9 system and factors influencing its efficacy for treating human diseases and in the field of agriculture, synthetic biology, and environmental sciences. The book also examines the recent advances in the CRISPR-Cas9 technology to improve crop productivity towards achieving the zero hunger goals. Further, the book discusses the recent developments and therapeutic applications of CRISPR-cas9 in gene therapy and the applications of CRISPR/Cas9 in situ correction of disease-causing mutations, loss-of-function mutations, or gain-of-function mutations in various genetic and neuromuscular diseases. It also explores the emerging opportunities of CRISPR-Cas9 in diagnosing and treating infectious diseases caused by bacterial, viral, protozoan, and other parasites. It aims to highlight the current landscape of the applications of CRISPR-Cas9 in the bioengineering and translational research of human diseases. It discusses the applications, mechanisms, strategies for utilizing the role of CRISPR/Cas 9 in engineering genetic circuits and metabolic pathways and metabolic remodeling.

Lastly, the book also reviews various biomedical, legal, and ethical issues related to CRISPR/Cas9 technology for its widespread utilization. It is an invaluable source of information for researchers interested in understanding the translational applications of CRISPR/Cas 9.

Contents:

Chapter 1: Evolution of CRISPR/Cas 9 as gene editing tool-Introduction chapter a) Brief History., b) Types of CRISPR/Cas system, c) Optimized gene knockout using CRISPR/Cas, d) CRISPR/Cas- based technologies.- Chapter 2: CRISPR/Cas 9 in prokaryotes: beyond adaptive immunity.- Chapter 3: Potential of CRISPR/Cas 9 in environmental and agriculture sciences.- Chapter 4: Clinical applications of CRISPR/Cas9 gene editing.- Chapter 5: CRISPR/Cas9-based strategies against infectious diseases.- Chapter 6: Diagnostics Application of CRISPR/Cas 9.- Chapter 7: CRISPR/Cas9 in biomedical engineering.- Chapter 8: CRISP/Cas: ethical and social issues.- Chapter 9: CRISPR/Cas: Challenges and future opportunities.