Main description:

This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution
Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang

2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection
Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye

3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors
Zhiyong Yang and Christopher D. C. Allen

4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements
Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya

5. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses
Katelyn M. Spillane and Pavel Tolar

6. Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments
Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, and Alexander Hoffmann

7. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells
Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, and Lilin Ye

8. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation
Xin Gao, Lin Lin, and Di Yu ;

9. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy
Anurag R. Mishral and Akanksha Chaturvedi

10. Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence Microscopy
Xiaoming Bai, Xiaodong Zhao, and Chaohong Liu

11. Imaging the Interactions between B Cells and Antigen-Presenting Cellsal,Jia C. Wang, Madison Bolger-Munro, and Michael R. Gold

12. In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes
Yolanda R. Carrasco

13. Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay
Kathrin Kla sener, Jianying Yang, and Michael Reth

14. Single Particle Tracking of Cell Surface Proteins
Laabiah Wasim and Bebhinn Treanor

15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen
Chung Park, Il-Young Hwang, and John H. Kehrl

16. Time-lapse Foerster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells
Hae Won Sohn and Joseph Brzostowski

17. Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System
Jing Wang, Zhengpeng Wan, and Wanli Liur

18. Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation
Heather Miller and Wenxia Song

19. Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation
Vid Sustar, Marika Vainio, Pieta K. Mattila