Main description:

Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units.

Contents:

Foreword| Ashok Pandey

Preface Arvind Bhatt, Ravi Bhatia, Tek Balla

Part I: Isolation, screening and culture maintenance

1. Isolation of microorganisms Mahinder Kumar Gupta

2. Screening strategies Chayanika Putatunda, Preeti Solanki, Shruti Pathania, Anil Kumar, and Abhishek Walia

3. Identification, morphological, biochemical, and genetic characterization of microorganisms Nivedita Sharma, Nisha Sharma, Shakshi Sharma, Pushpinder Sharma, and Bindu Devi

4. Microbial activity and productivity enhancement strategies Shashi Kant Bhatia, Vijay Kumar, Virender Kumar, Ravi Kant Bhatia, and Yung-Hun Yang

5. Culture maintenance, preservation, and strain improvement Aman Kumar, Srijana Mukhia, Anil Kumar, Kiran Dindhoria, Neha Baliyan, and Rakshak Kumar

Part II: Laboratory techniques & instrumentation

6. Biomolecules: Types, homogenization, bead beater, sonication Arshad Jawed

7. Centrifugation: Basic principle, types Gaurav Sood, Minakshi Sharma, and Rajesh Kaushal

8. Spectroscopy-Principle, types, and applications Sweta Sinha, Christine Jeyaseelan, Gunjan Singh, Tanya Munjal, and Debarati Paul

9. Protein purification: Basic principles and techniques Alla Singh, Krishan Kumar, Dharam Paul Chaudhary, Neeraj Kumar, and Deepak Pandey

10. Chromatography: Basic principle, types, and applications Mahesh Kumar Gupta and Pradip Kumar Biswas

11. Electrophoresis: Basic principle, types, and applications Babita Rana and Gopal Krishna Joshi

Part III: Genomic and proteomic analysis

12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis Rupali Sharma, Shashwat Sharad, Gillipsie Minhas, Deep Raj Sharma, Kulsajan Bhatia, and Neel Kamal Sharma

13. RDT and genetic engineering: Basic of RDT method, PCR, and application Komaljeet Gill, Shivanti Negi, Neerja Rana, and Pankaj Kumar

14. Protein sequence analysis Deepak Sharma and Abhishek Chaudhary

15. Computational strategies and tools for protein tertiary structure prediction Raj Kumar and Ajay Sharma

16. Docking strategies Arushi Sharma and Ragothaman M. Yennamalli

17. A beginner's guide to measuring binding affinity during biomolecular interactions Hannah I. Martin, Vidushi Agnihotri, Ragothaman M. Yennamalli, and Aurijit Sarkar

Part IV: Industrially important enzymes

18. Enzymes and their significance in the industrial bioprocesses Neerja Thakur, Sheetal, and Tek Chand Bhalla

19. Enzyme kinetics: Industrially important enzymes Archana Singh, Pranjali Singh, Ankit Singh, Deepak Pandey, Durgavati Yadav, and Kumar Sandeep

20. Industrial enzymes: Basic information, assay, and applications Jagdish Singh and Parmjit S. Panesar

Part V: Techniques in process development

21. Fundamentals of fermentation technology Manya Behl, Saurabh Thakar, Hemant Ghai, Deepak Sakhuja, and Arvind Kumar Bhatt

22. Sterilization in bioprocesses Sampan Attri and Gunjan Goel

23. Scale-up: Lab to commercial scale Krunal Dholiya, Ashesh Parmar, and Hafiza Sidra Bashir

24. Bioprocess: Control, management, and biosafety issues Subhasish Dutta and Debanko Das

25. Demonstration and industrial scale-up Govindarajan Ramadoss, Saravanan Ramiah Shanmugam, and Thirupathi Kumara Raja Selvaraj

26. Downstream processing of biotechnology products Dattatray Bedade and Shweta Pawar

27. Waste management and environment Aakarsha Handa and Paulraj Rajamani

Part VI: Feasibility, marketing and biobusiness

28. Biobusiness opportunities Ashutosh Gupta and Gitika Nagrath

29. Feasibility analysis and business plan Ashok Kumar Bansal

30. Patenting, IPR, and regulatory issues Sarita Devi

31. Commercialization and technology transfers of bioprocess Amit Seth, Aditya Banyal, and Pradeep Kumar

32. Bioproduct marketing strategies Shivani Chauhan, Prakriti Jhilta, and Ravi Kant Bhatia