Main description:

Unique in scope and approach, this comprehensive text on analytical methods in modern biosciences presents a thorough introduction into the physical and chemical background as well as a discussion of the strengths and weakness for each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids such as DNA and RNA. By its carefully coordinated method descriptions and frequent cross-references showing the connections between different techniques, the reader will gain a thorough overview over the entire subject and will be able to select the most appropriate method for any given bioanalytical challenge, making the text an invaluable resource for all students and researchers tasked with setting up and evaluating experimental research.
The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Optical spectroscopy - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic techniques - Gel and capillary electrophoresis - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - NMR and EPR of biomolecules - X-ray structure analysis - Carbohydrate analysis - Lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Genomic analysis - Proteome and metabolome analysis - Topnomics, interactomics and chemical biology

Contents:

Content Preface 1. Bioanalysis 1.1 Paradigm change in Biochemistry: from protein biochemistry to systems biology 1.2 Methods as driver in scientific progress 2. Protein purification 3. Protein determination 4. Enzymatic activity testing 5. Microcalorimetry 6. Immunologic techniques 7. Chemical modification of proteins and protein complexes 8. Spectroscopy 9. Microscopy ? Imaging 10. Cleavage of proteins 11. Chromatographic separation methods 12. Electrophoretic techniques 13. Capillary electrophoresis 14. Amino acid analysis 15. Protein sequence analysis 16. Mass spectrometry 17. Protein-protein interactions 18. Biosensors 19. Magnetic resonance spectroscopy of biomolecules 20. Electron microscopy 21. Scanning force microscopy 22. X-Ray structure analysis 23. Analysis of synthetic peptides 24. Carbohydrate analysis 25. Lipid analysis 26. Analysis of posttranslational modifications 27. Isolation and Purification of nucleic acids 28. Preparation of nucleic acids 29. Techniques for the Hybridization and Detection of Nucleic Acids 30. PCR 31. DNA Sequence analysis 32. Analysis of epigenetic modifications 33. Protein-nucleic acid interactions 34. Sequence analysis 35. Analysis of active RNA synthesis 36. Hybridization of fluorescent DNA in genome analysis in molecular cytogenetic 37. Physical and genetic gene mapping 38. Differential gen activity 39. DNA microarray technology 40. The Use of Oligonucleotides as Tools in Cell Biology 41. Proteome analysis 42. Metabolomics and Peptidomics 43. Interactomics ? systematic protein-protein interactions 44. Chemical Biology 45. Toponom analysis