Main description:

Based on a very successful handbook as well as student lectures given by the author, this textbook comprehensively covers the theory, methods and applications of protein engineering and biocatalysis. The author is well known due to his strong background in industry as well as academia, and has extensive teaching experience in Europe and North America. From the contents: Basics Handling of enzyme biocatalysts Protein engineering Stabilization and retention of biocatalysts Applications Specialties Of interest to a wide audience of (bio)chemists and bioengineers.

Contents:

I. basics 1. key features of biocatalysis; comparison with other catalysts 2. protein biochemistry: protein folding, enzyme mechanisms 3. activity, selectivity, and stability of protein biocatalysts II. handling of enzyme biocatalysts 4. isolation and purification 5. kinetics 6. enzyme reactors III. protein engineering 7. rational methods 8. combinatorial methods 9. data-driven methods IV. stabilization and retention of biocatalysts 10. formulation in aqueous systems 11. immobilization on solid support 12. enzymes in organic solvents and other nonaqueous media V. applications 13. enzymes as products: detergents, textiles, pulp and paper, animal feed 14. enzymes as catalysts: chiral intermediates, active pharma ingredients 15. enzymes as components in fermentation systems: biofuels, pharma compounds VI. specialities 16. evolution and creation of novel enzyme activities 17. artificial protein catalysts 18. metabolic engineering