Main description:

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Contents:

Part I: In Vitro/Ex Vivo Imaging Applications

1. High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines

Maria Maddalena Calabretta, Laura Montali, Antonia Lopreside, Elisa Michelini, and Aldo Roda

2. Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells

Setsuko Tsuboi and Takashi Jin

3. High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays

Tingting Xu, Anna Young, Jasleen Narula, Gary Sayler, and Steven Ripp

4. Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon

Carola Gregor

5. Bioluminescent Imaging and Tracking of Bacterial Transport in Soils

Jie Zhuang, Weipeng Liu, Liqiong Yang, Jia Kang, and Xiaoming Zhang

Part II: In Vivo Imaging of Microbial Pathogenesis

6. In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

Jose A. Guijarro and Jessica Mendez

7. In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

Diana Caridha, Susan Leed, and Alicia Cawlfield

8. Multiplex Imaging of Polymicrobial Communities - Murine Models to Study Oral Microbiome Interactions

Jens Kreth, Yasser M. Abdelrahman, and Justin Merritt

9. Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivo

Katrien Van Dyck , Patrick Van Dijck, and Greetje Vande Velde

Part III: In Vivo Small Animal/Organism Imaging Applications

10. Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson's Disease

Maria Eugenia Bernis and Gultekin Tamguney

11. Continual Conscious Bioluminescent Imaging in Freely Moving Mice Juan Antinao Diaz, Amy Geard, Lorna M. FitzPatrick, Juliette M.K.M Delhove, Suzanne M.K. Buckley, Simon N. Waddington, Tristan R. McKay, and Rajvinder Karda

12. In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model

Shan Lei, Weijin Huang, Youchun Wang, and Qiang Liu

13. Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse Model

Derek Yip, Andrew Kirkpatrick, Tingting Xu, Tom Masi, Stacy Stephenson, Steven Ripp, and Dan Close

14. In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice

Prakash Gangadaran and Byeong-Cheol Ahn

15. Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay

Benedikt Jefferies, Zhichao Tong, and Roman Nawroth

16. Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models

Xiang Li and Minyong Li

Part IV: In Planta Imaging Applications

17. The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells

Tomoaki Muranaka and Tokitaka Oyama