Main description:

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.

Contents:

Part I: Invertebrate Models

1. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra

Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, and Brigitte Galliot

2. Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis

Jamie A. Havrilak and Michael J. Layden

3. Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis

Fabian Rentzsch, Eduard Renfer, and Ulrich Technau

4. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

Elena Perea-Atienza, Brenda Gavilan, Simon G. Sprecher, and Pedro Martinez

5. Immunostaining of the Embryonic and Larval Drosophila Brain

Frank Hirth and Danielle C. Diaper

6. Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

David Jussen and Rolf Urbach

7. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

Karoline F. Kraft and Rolf Urbach

8. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update

Emma L. Powell and Iris Salecker

9. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

Karolina Miszczak and Boris Egger

10. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila

Cornelia Fritsch and Simon G. Sprecher

11. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development

Max S. Farnworth, Kolja N. Eckermann, Hassan M.M. Ahmed, Dominik S. Muhlen, Bicheng He, and Gregor Bucher

12. A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum

Marita Buscher, Georg Oberhofer, Natalia Carolina Garcia-Perez, and Gregor Bucher

13. Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium

Vera S. Hunnekuhl, Max S. Farnworth, Bicheng He, and Gregor Bucher

14. X-Ray Microscopy of the Larval Crustacean Brain

Jakob Krieger and Franziska Spitzner

15. Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain

Steffen Harzsch and Caroline Viertel

16. Immunostainings in Nervous System Development of the Nematode C. elegans

Janet S. Duerr

17. Methods in Brain Development of Molluscs

Andreas Wanninger and Tim Wollesen

18. A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization

Clare Hudson

19. Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum

Yann Le Petillon, Stephanie Bertrand, and Hector Escriva

Part II: Vertebrate Models

20. In Situ Hybridization and Immunostaining of Xenopus Brain

Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, and Rong-qiao He

21. Morpholino Studies in Xenopus Brain Development

Jennifer E. Bestman and Hollis T. Cline

22. Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish

Gilbert Lauter, Iris Soell, and Giselbert Hauptmann

23. Live Morphometric Classification of Sensory Neurons in Larval Zebrafish

Gema Valera and Hernan Lopez-Schier

24. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain

Richard P. Tucker, Tatsuto Ishimaru, and Qizhi Gong

25. Gene Silencing in Chicken Brain Development

Georgia Tsapara, Irwin Andermatt, and Esther T. Stoeckli

26. Transplantation of Neural Tissue: Quail-Chick Chimeras

Andrea Streit and Claudio D. Stern

27. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

Jinling Liu and Aimin Liu

28. The Cre/Lox System to Assess the Development of the Mouse Brain

Claudius F. Kratochwil and Filippo M. Rijli

29. In Utero Electroporation to Study Mouse Brain Development

Emilie Pacary and Francois Guillemot