Main description:

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model
Emma K. Hollmann, Yajuan Shi, and Ethan S. Lippmann

2. In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models
Anke M. Tukker, Fiona M.J. Wijnolts, Aart de Groot, Richard W. Wubbolts, and Remco H.S. Westerink

3. Oxidative Stress Signatures in Human Stem Cell Derived Neurons
M. Diana Neely and Aaron B. Bowman

4. Glial Reactivity in Response to Neurotoxins: Relevance and Methods
Lindsay T. Michalovicz and James P. O'Callaghan

5. Neuron-Glia Interaction Studied with In Vitro Co-Culture
Mancino S., Serafini MM., and Viviani B.

6. Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria
Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, and Jeremy W. Chambers

7. Epigenetic Changes in Cultured Neurons and Astrocytes
David P. Gavin, Xiaolu Zhang, and M. Guizzetti

8. The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation
Laura Nimtz, Joerdis Klose, Stefan Masjosthusmann, Marta Barenys, and Ellen Fritsche

9. Zebrafish as a Tool to Assess Developmental Neurotoxicity
Keturah G. Kiper and Jennifer L. Freeman

10. Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies
Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya Lopez, Edgar Rangel- Lopez, and Abel Santamaria

11. Electrophysiological Neuromethodologies
Yukun Yuan and William D. Atchinson

12. A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies
Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller, James P. O'Callaghan, Serguei Liachenko, Merle G. Paule, and Syed Z. Imam

13. Transporter Studies: Brain Punching Technique
Cherish A. Taylor and Somshuvra Mukhopadhyay

14. MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders
Lena Smirnova and Alexandra Maertens

15. Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes
Anna Bal-Price and Francesca Pistollato