Main description:

This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of "naked" CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

Contents:

PART I: Introduction, selection, mechanisms.

1. Cell-penetrating peptides

Matjaz Zorko and UElo Langel

2. Shuttling homeoproteins and their biological significance

Alain Prochiantz and Ariel Di Nardo

3. Artificial evolutionary optimization process to improve the functionality of CPPs

Niels Roeckendorf, Katrin Ramaker, and Andreas Frey

4. In silico design of chemically modified cell penetrating peptides

Vinod Kumar, Gajendra Pal Singh Raghava

5. Synthetic Molecular Evolution of Cell Penetrating Peptides

William C. Wimley

6. A native CPP from rattlesnake with therapeutic and theranostic properties

Lucas C. Porta, Joana D'Arc Campeiro, and Mirian A. F. Hayashi

7. Synthesis of cell-penetrating peptide coated silica nanoparticles and their physicochemical and biological characterization

Isabel Gessner, Annika Klimpel and Ines Neundorf

8. Enhancing the therapeutic potential of extracellular vesicles using peptide technology

Carla Martin-Perez, Mariana Conceicao, Richard Raz, Matthew J. A. Wood, and Thomas C. Roberts

9. Challenges and methods for the study of CPP translocation mechanisms

Astrid Walrant, Francoise Illien, Sandrine Sagan, and Nicolas Rodriguez

10. Membrane molecular interactions and induced structures of CPPs

Fatemeh Madani and Astrid Graslund

11. A single GUV method for revealing the action of cell-penetrating peptides in biomembranes

Md. Mizanur Rahman Moghal, Madhabi Lata Shuma, Md. Zahidul Islam, and Masahito Yamazaki

12. Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptides

Emerson R.Silva, Lucas R. Mello, and Ian Hamley

13. Imaging CPP delivery mechanisms of oligonucleotides

Najoua El Boujnouni, Alexander H. van Asbeck, Jurgen Dieker, Derick G. Wansink, and Roland Brock

14. Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in non-adherent cells

Edward J. Sayers, Victoria L. Barlow, Yu-Hsuan Tsai, and Arwyn T. Jones

15. PepFect14: signaling and transfection

Luca Falato, Maxime Gestin, and Ulo Langel

16. Utilisation of cell-penetrating peptides for in vivo delivery of bioactive cargo - the effect of nanoparticle formulation

Piret Arukuusk, Heleri Heike Hark, and UElo Langel

Part II: Delivery of proteins and protein mimics.

17. Protein delivery by PTDs/CPPs

Caroline Palm-Apergi and Steve F. Dowdy

18. Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing a Tandem Repeat Penetration-Accelerating Sequence

Akiko Okuda and Shiroh Futaki

19.Recombinant transcription factors (TFs) fused to ZEBRA minimal transduction domain (MD) for modulation of mRNA transcripts.

Benjamin Caulier, Jean-Luc Lenormand, and David Laurin

20. Protein mimicry and the design of bioactive cell penetrating peptides: The genesis of STOPSPERM bioportides

John Howl*, Joana Vieira Silva,, Margarida Fardilha, and Sarah Jones

21. Lipopeptide pepducins as therapeutic agents

Emily Michael, Lidija Covic, and Athan Kuliopulos

22. Delivery of Antibiotics by Cell-Penetrating Peptides to Kill Intracellular Pathogens

Christian Ruter

23. CPP applications in immune modulation and disease therapy

Ja-Hyun Koo, Won-Ju Kim, and Je-Min Choi

Part III: Targeting

24. Cell-penetrating peptides as carriers for transepithelial drug delivery

Danai Anastasia Panou, Ragna Guldsmed Diedrichsen, Mie Kristensen, and Hanne Morck Nielsen

25. New tools for streamlined in vivo homing peptide identification

Kristina Posnograjeva, Karlis Pleiko, Maarja Haugas, and Tambet Teesalu

26. Tumor targeting as new therapeutic approach

Eric Savier, Pierre Tuffery, Heriberto Bruzzoni-Giovannelli and Angelita Rebollo

27. Mitochondrial Targeting Probes, drug conjugates and gene therapeutics

Carmine Pasquale Cerrato, Tove Kivijarvi, and UElo Langel

28. Novel brain penetrating single chain antibodies directed against 3RTau for the Treatment of Alzheimer's Disease and related Dementias

Brian Spencer, Robert A. Rissman, Cassia Overk, and Eliezer Masliah

29. Strategies for peptide-mediated cargo delivery to human smooth muscle cells

Leo Gurney, Steven C. Robson, Michele Sweeney, Arwyn T. Jones, and Michael J. Taggart

Part IV: Oligonucleotide delivery

30. Tips and Tools to Understand Direct Membrane Translocation of siRNA-Loaded WRAP-Based Nanoparticles.

Sebastien Deshayes, Karidia Konate, Eric Vives, and Prisca Boisguerin

31. Evaluating efficacy of peptide-delivered oligonucleotides using the severe Taiwanese SMA mouse model

Larissa Goli, Jessica Stoodley, Suzan Hammond, and Richard Raz

32. Development of CPP-based methods for delivery of miRNAs into the skin and airways - lessons from cell culture and mouse models

Anet Laanesoo, Kapilraj Periyasamy, Margus Pooga, and Ana Rebane

33. Endpoint and kinetic approaches for assessing transfection efficacy in mammalian cell culture

Ly Porosk, Jekaterina Nebogatova, Ilja Gaidutsik, and UElo Langel

34. Tissue analysis of lung-targeted delivery of siRNA and plasmid DNA

Kaido Kurrikoff, Birgit Vunk, and UElo Langel

35. Improvement of transfection with PepFects by organic and inorganic components.

Moataz Dowaidar, Hani Nasser Abdelhamid, and Ulo Langel

36. DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent

Kristi Kasemaa, Kaia Talts, Evelin Taht, Urve Toots, and Mart Ustav

37. Use of PepFect14 and siRNA for targeted gene silencing in human embryonic stem cells

Egle-Helene Ervin, Margus Pooga, and Toivo Maimets

38. CRISPR/Cas9 plasmid delivery through the CPP: PepFect14

Luca Falato, Birgit Vunk, and UElo Langel

39. Applications of CPPs in genome editing of plants

Atta Soliman, John Laurie, Andriy Bilichak, and Alicja Ziemienowicz

40. Live Cell Genomics: Cell-specific transcriptome capture and exon-specific RNA binding protein isolation in live tissues and cells

Jaehee Lee and James Eberwine