Main description:

This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naive and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Human Donor Cell Types: Potency and Position
1. Frontiers of PluripotencyAlejandro De Los Angeles
2. Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions Alejandro De Los Angeles, Daiji Okamura, and Jun Wu
3. Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem CellsRyohichi Sugimura
4. Cancer Stem Cells: Concepts, Challenges and Opportunities for Cancer TherapyMay Yin Lee, Rajshekhar R. Giraddi, and Wai Leong Tam
5. Informed Consent Issues for Cell DonorsInsoo Hyun
Part II Non-Human Hosts: Species and Developmental Stages 6. Chick Models and Human-Chick Organizer GraftsIain Martyn, Tatiane Y. Kanno, and Ali H. Brivanlou 7. The Engraftment of Lentiviral Vector-Transduced Human CD34+ Cells into Humanized MiceYoon-Sang Kim, Matthew Wielgosz, and Byoung Ryu 8. Pig Chimeric Model with Human Pluripotent Stem Cells Cuiqing Zhong, Jun Wu, and Juan Carlos Izpisua Belmonte
9. Embryonic Chimeras with Human Pluripotent Stem CellsAlejandro De Los Angeles, Masahiro Sakurai, and Jun Wu 10. Neural Stem Cell Transplantation into a Mouse Model of Stroke Alejandro De Los Angeles
11. Ethical Standards for Chimera Research OversightInsoo Hyun Part III New Directions and Controversies
12. Ethical Considerations in Crossing the XenobarrierSebastian Porsdam Mann, Rosa Sun, and Goeran Hermeren
13. Neurological Chimeras and the Moral StaircaseDaniel Counihan
14. Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells Nilam Sinha, Eoin C. Whelan, and Ralph L. Brinster
15. Human-Monkey Chimeras for Modeling Human Disease: Opportunities and ChallengesAlejandro De Los Angeles, Insoo Hyun, Stephen R. Latham, John D. Elsworth, and D. Eugene Redmond Jr.