Main description:

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry.

Contents:

1. Recognition Mechanisms of Chiral Selectors: An Overview

Gerhard K.E. Scriba

2. Enantioseparation by Thin Layer Chromatography

Rituraj Dubey and Ravi Bhushan

3. Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase

Sheng-Ming Xie, Jun-Hui Zhang, and Li-Ming Yuan

4. Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS

Toshimasa Toyo'oka

5. Chiral Mobile Phase Additives in HPLC Enantioseparations

Lushan Yu, Shengjia Wang, and Su Zeng

6. Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High Performance Liquid Chromatography: An Overview

Bezhan Chankvetadze

7. HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions

Roberto Cirilli

8. Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC

Jie Zhou, Jian Tang, and Weihua Tang

9. HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases

Xiaoxuan Li and Yong Wang

10. Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector

Liang Zhao, Hui Li, Shuqing Dong, and Yanping Shi

11. Cyclofructans as Chiral Selectors: An Overview

Garrett Hellinghausen and Daniel W. Armstrong

12. High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview

Istvan Ilisz, Timea Orosz, and Antal Peter

13. Application of Sub-2 Micron Particles Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography

Chiara Fanali and Salvatore Fanali

14. Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview

Istvan Ilisz, Attila Bajtai, Antal Peter, and Wolfgang Lindner

15. Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand-Exchange

Federica Ianni, Lucia Pucciarini, Andrea Carotti, Roccaldo Sardella, and Benedetto Natalini

16. Applications of Chiral Supercritical Fluid Chromatography

Emmanuelle Lipka

17. Chiral Separations by Countercurrent Chromatography

Sheng-Qiang Tong

18. Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations

Gerhard K.E. Scriba and Pavel Jac

19. Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations

Anne-Catherine Servais and Marianne Fillet

20. Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer

Joachim Wahl and Ulrike Holzgrabe

21. Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins

Anne-Catherine Servais and Marianne Fillet

22. Chiral Separation by NACE Using Polyol Derivative-Boric Acid Complexes

Lijuan Wang, Xu Hou, Fan Zhang, Ying Liu, Yimeng Ren, and Hongyuan Yan

23. Chiral Capillary Electrophoresis-Mass Spectrometry

Maria Castro-Puyana and Maria Luisa Marina

24. Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography

Wan Aini Wan Ibrahim, Siti Munirah Abd Wahib, Dadan Hermawan, and Mohd Marsin Sanagi

25. Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry

Vijay Patel and Shahab A. Shamsi

26. Application of a (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography

Wonjae Lee, Kyung Tae Kim, and Jong Seong Kang

27. Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview

Luiz Carlos Klein-Junior, Debby Mangelings, and Yvan Vander Heyden