Main description:

This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Overview
1. An Overview of CE in Clinical AnalysisDavid S. Hage
Part II Applications in Clinical Chemistry
2. Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological StudiesPetr Tuma
3. Monitorization of 1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF DetectionMaria del Mar Barrios-Romero, Agustin G. Crevillen, Angel Puerta, Mercedes de Frutos, and Jose Carlos Diez-Masa
4. Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic InjectionChenhua Zhang, William Clarke, and David S. Hage
5. On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum TransthyretinRoger Pero-Gascon, Laura Pont, Victoria Sanz-Nebot, and Fernando Benavente
6. Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid ArthritisChristian Schwedler and Veronique Blanchard
7. The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity DetectionPetr Tuma
8. Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization Morten E. Pedersen, Jesper Ostergaard, and Henrik Jensen
Part III Application in Drug Analysis
9. A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of ExperimentHassan Y. Aboul-Enein and Ahmed M. Abdel-Megied
Part IV Application in Metabolomics
10. New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS)Meera Shanmuganathan and Philip Britz-McKibbin
11. Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited SampleWei Zhang, Thomas Hankemeier, and Rawi Ramautar
Part V Applications in Paediatrics
12. Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip ElectrophoresisAnna V. Nielsen and Adam T. Woolley
13. Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary ElectrophoresisTerry M. Phillips and Edward F. Wellner
14. Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat HyperexpansionsIndhu-Shree Rajan-Babu and Samuel S. Chong
Part VI Applications in Oncology
15. A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole BloodAngelo Zinellu, Elisabetta Sotgiu, Salvatore Sotgia, and Ciriaco Carru
16. Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA)Noemi Farina-Gomez, Diana Navarro-Calderon, Angel Puerta, Monica Gonzalez, Jose Carlos Diez-Masa, and Mercedes de Frutos
17. Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate ElectrophoresisTijl Vermassen, Nico Callewaert, Sylvie Rottey, and Joris R. Delanghe 18. Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic AntigenShulin Zhao
Part VII Application in Virology
19. Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins Chao-Xuan Zhang and Michael M. Meagher