Main description:

This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.

Contents:

1. Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory

Y. Victoria Zhang and Uttam Garg

2. System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Shannon Haymond

3. Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders

David Scott, C. Clinton Frazee III, Bryce Heese, and Uttam Garg

4. Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

J. Grace Van Der Gugten and Daniel T. Holmes

6. Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism

Stephen M. Roper, Annette L. Weindel, and Dennis J. Dietzen

7. Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS)

Hamed Piri-Moghadam, Alan Miller, Debra Pronger, Faye Vicente, Joel Charrow, Shannon Haymond, and David C. Lin

8. Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)

Judith A. Hobert, Stephen A. Brose, and Marzia Pasquali

9. Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry

Serena Baird, C. Clint Frazee III, and Uttam Garg

10. Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode

Wayne B. Anderson, Putuma P. Gqamana, and Y. Victoria Zhang

11. Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS

Julie A. Ray, Erik Kish-Trier, and Lisa M. Johnson

12. Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine

Ning Liu and Qin Sun

13. Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS

Ryan C. Schofield, Daniel Kirchoff, and Dean C. Carlow

14. Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry

Erik Kish-Trier and Tatiana Yuzyuk

15. Quantitation of -Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

16. A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens

Vrajesh Pandya and Elizabeth L. Frank

17. Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS)

Melissa Beals, Bheemraj Ramoo, C. Clinton Frazee III, and Uttam Garg

18. Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Heather A. Nelson and Elizabeth L. Frank

19. Quantitation of IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

J. Grace Van Der Gugten, Daniel T. Holmes, and Andre Mattman

20. Quantification of Insulin Analogs by Liquid Chromatography-High Resolution Mass Spectrometry

Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh

21. Differentiation of Common IGF-1 Variants Using HRAM-MS COM Determination with Follow-Up MS/MS Verification

Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh

22. Monitoring and Identifying Insulin-Like Growth Factor 1 Variants by Liquid Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory

Ievgen Motorykin, Allison Li, and Zengru Wu

23. Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry

Brandi Wasek and Erland Arning

24. Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry

Hsuan-Chieh (Joyce) Liao and Hsiao-Jan Chen

25. Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem Mass Spectrometry

Brandon B. Stauffer and Chunli Yu

26. Detection of 13C-Mannitol and Other Saccharides Using Tandem Mass Spectrometry for Evaluation of Intestinal Permeability, or Leaky Gut

Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh

27. LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers without Chromatographic Separation

Mark M. Kushnir, Gordon J. Nelson, Elizabeth L. Frank, and Alan L. Rockwood

28. Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

29. Screening of Organic Acidurias by Gas Chromatography Mass Spectrometry (GC-MS)

David Scott, C. Clinton Frazee III, and Uttam Garg

30. Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC/MS)

Stanley F. Lo, Keely Pierzchalski, Velta Young, and William J. Rhead

31. Quantitative Organic Acids in Urine by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GCxGC-TOFMS)

Erland Arning, Paula Ashcraft, Jeanna Bennett-Firmin, and Lawrence Sweetman

32. Quantification of Parathyroid Hormone and Its Fragments in Serum by Liquid Chromatography-High Resolution Mass Spectrometry

Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh

33. High Sensitivity Measurement of Parathyroid Hormone Related Protein (PTHrP) in Plasma by LC-MS/MS

Mark M. Kushnir and Alan L. Rockwood

34. Quantitation of Phenylalanine in Dried Blood Spot using Liquid Chromatography Tandem Mass Spectrometry for Monitoring of Patients with Phenylketonuria (PKU)

Serena Baird, C. Clinton Frazee III, and Uttam Garg

35. An Optimized Procedure for Proteomic Analysis of Extracellular Vesicles Using In-Stage Tip Digestion and DIA LC-MS/MS: Application to Liquid Biopsy in Cancer

Rajesh K. Soni, Lyssa Dimapanat, Manpreet S. Katari, and Alex J. Rai

36. High-Throughput Plasma Proteomic Profiling

Rajesh Kumar Soni

37. Quantitation of Purine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Qin Sun

38. Quantitation of Pyrimidine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Ning Liu and Qin Sun

39. Quantitation of Renin Activity in Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

J. Grace Van Der Gugten and Daniel T. Holmes

40. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone

Ada Munar, C. Clinton Frazee III, and Uttam Garg

41. A User-Friendly Sample Preparation Alternative for Manual and Automated LC-MS/MS Quantification of Testosterone

Judy A. Stone, Dawn Francisco, Heather Tone, Joshua Akin, and Robert L. Fitzgerald

42. Quantitation of Thyroglobulin in Serum Using SISCAPA and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

J. Grace Van Der Gugten, Morty Razavi, and Daniel T. Holmes

43. Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry

Weijia (William) Wu, Michael J. McPhaul, and Zengru Wu

44. Quantification of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry

Serena Baird, C. Clinton Frazee III, and Uttam Garg

45. Very Long-Chain Fatty Acids Quantification by Gas-Chromatography Mass Spectrometry

Anna I. Scott

46. Quantification of Very Long-Chain and Branched-Chain Fatty Acids in Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Irene De Biase and Marzia Pasquali

47. High Throughput Analysis of 25-OH-Vitamin D2 and D3 Using Multi-Channel Liquid Chromatography-Tandem Mass Spectrometry

Putuma P. Gqamana and Y. Victoria Zhang

48. Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid Chromatography-Tandem Mass Spectrometry

Ada Munar, Clinton Frazee III, and Uttam Garg