Main description:

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

Contents:

Part I: Clinical Metabolomics and Lipidomics

1. Metabolomics as a Tool to Understand Pathophysiological Processes

Julijana Ivanisevic and Aurelien Thomas

2. Metabolomics in Immunology Research

Bart Everts

Part II: LC-MS-Based Metabolomics

3. LC-MS-Based Metabolomics of Biofluids Using All Ion Fragmentation (AIF) Acquisition

Romanas Chaleckis, Shama Naz, Isabel Meisterll, and Craig E. Wheelock 4. Lipid Mediator Metabolomics via LC-MS/MS Profiling and Analysis

Jesmond Dalli, Romain A. Colas, Mary E. Walker, and Charles N. Serhan

5. UHPSFC/ESI-MS Analysis of Lipids

Miroslav Lisa and Michal Holcapek

6. LC-MS/MS Analysis of Lipid Oxidation Products in Blood and Tissue Samples

Yiu Yiu Lee and Jetty Chung-Yung Lee

7. Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum

8. LC-MS/MS Analysis of Bile Acids

Sabrina Krautbauer and Gerhard Liebisch

9. LC-MS/MS Analysis of Triglycerides in Blood-Derived Samples

Madlen Sander, Susen Becker, and Uta Ceglarek

10. LC-MS/MS Analysis of the Epoxides and Diols Derived from the Endocannabinoid Arachidonoyl Ethanolamide

Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, and Christophe Morisseau 11. Sphingolipid Analysis in Clinical Research

Bo Burla, Sneha Muralidharan, Markus R. Wenk, and Federico Torta

12. Shotgun Lipidomics Approach for Clinical Samples

Lars F. Eggers and Dominik Schwudke

13. Establishing and Performing Targeted Multi-Residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples

Theresa L. Pedersen and John W. Newman

14. Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis

Wei Han and Liang Li

15. Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma & Serum Using the LipidyzerTM Platform

Baljit K. Ubhi

Part III: GC-MS-Based Metabolomics

16. Exploratory GC-MS-Based Metabolomics of Body Fluids

Carole Migne, Stephanie Durand, and Estelle Pujos-Guillot

17. GC-MS Analysis of Short-Chain Fatty Acids in Feces, Cecum Content, and Blood-Derived Samples

Lisa Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, and Martin Giera 18. GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples

Lisa Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, and Martin Giera

19. Analysis of Oxysterols

Fabien Riols and Justine Bertrand-Michel

20. Analysis of Metabolites from the Tricarboxylic Acid Cycle for Yeast and Bacteria Samples Using Gas Chromatography Mass Spectrometry

Reza Maleki Seifar, Angela ten Pierick, and Patricia T.N. van Dam

21. GC-MS Analysis of Lipid Oxidation Products in Blood, Urine, and Tissue Samples

Anne Barden and Trevor A. Mori

Part IV: CE-MS-Based Metabolomics

22. Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-Covalently Coated Capillaries

Rawi Ramautar

23. CE-MS for the Analysis of Amino Acids

Karina Trevisan Rodrigues, Marina Franco Maggi Tavares, and Ann Van Schepdael

Part V: NMR-Based Metabolomics

24. NMR Analysis of Fecal Samples

Hye Kyong Kim, Sarantos Kostidis, and Young Hae Choi

25. Quantitative Analysis of Central Energy Metabolism in Cell Culture Samples

Sarantos Kostidis

Part VI: MALDI-Based Techniques and Mass Spectrometry Imaging of Clinical Samples

26. Mass Spectrometry Imaging of Metabolites

Benjamin Balluff and Liam A. McDonnell Part VII: Study Design, Data Analysis, and Bioinformatics

27. Quality Assured Biobanking: The Leiden University Medical Center Model

Rianne Haumann and Hein W. Verspaget

28. Extracting Knowledge from MS Clinical Metabolomic Data: Processing and Analysis Strategies

Julien Boccard and Serge Rudaz