Main description:

This volume explores the latest techniques in inner ear development, analysis of its sensory cells, and characterization and manipulation of the central auditory and vestibular pathways. The chapters in this book cover topics such as dissection and imaging of the cochlea; behavioral evaluation of animal models of diseases like tinnitus; hair cell function and regeneration; and recent advances in sequencing technology. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Developmental, Physiological, and Function Neurobiology of the Inner Ear is a valuable resource for scientists and researchers interested in learning more about this developing field.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

Part I Experimental Manipulation of the Inner Ear

1. Pou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Post-Natal Mice
Jennifer S. Stone, Edwin W. Rubel, and Mark E. Warchol

2. Cochlear Explant Cultures: Creation and Application
Elizabeth Carroll Driver and Matthew W. Kelley

3. Immunohistochemistry and In Situ mRNA Detection using Inner Ear Vibratome Sections
Mirko Scheibinger, Amanda Janesick, Giovanni H. Diaz, and Stefan Heller

4. Genetic Manipulation of the Embryonic Chicken Inner Ear
Nicolas Daudet, Magdalena Zak, Thea Stole, and Stephen Terry

5. Molecular Tools to Study Regeneration of the Avian Cochlea and Utricle
Amanda Janesick, Mirko Scheibinger, and Stefan Heller

Part II Molecular Analysis of the Inner Ear

6. An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization
Sumana Ghosh, Graham Casey, Kendra L. Stansak, Punam Thapa, and Bradley J. Walters

7. A Manual Technique for Isolation and Single-Cell RNA Sequencing Analysis of Cochlear Hair Cells and Supporting Cells
Cody West, Paul T. Ranum, Ryotaro Omichi, Yoichiro Iwasa, Daniel Walls, Jin-Young Koh, and Richard J.H. Smith

8. Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types
Maggie S. Matern, Beatrice Milon, Ran Elkon, and Ronna Hertzano

Part III Hair Cell Function and Physiology

9. In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Function
Saman Hussain, Roberto Aponte-Rivera, Rana M. Barghout, Josef G. Trapani, and Katie Kindt

10. Electrophysiological Recordings of Voltage Dependent and Mechanosensitive Currents in Sensory Hair Cell of the Auditory and Vestibular Organs of the Mouse
Artur A. Indzhykulian, Stuart L. Johnson, and Gwenaelle S.G. Geleoc

11. Biophysical Recording from Adult Hair Cells
Antonio Miguel Garcia de Diego and Jonathan F. Ashmore

Part IV The Central Auditory Pathway

12. Endocochlear Potential Measures, Local Drug Application, and Perilymph Sampling in the Mouse Inner Ear
Kevin K. Ohlemiller, Jared J. Hartsock, and Alec N. Salt

13. In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus
Hsin-Wei Lu and Philip Joris

14. Measurement of Human Cochlear and Auditory Nerve Potentials
Eric Verschooten and Philip X. Joris

15. Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function using Electrical Stimulation and Optogenetics
Kirupa Suthakar, Lester Torres Cadenas, and Catherine Weisz

16. Auditory Brainstem Response (ABR) Measurements in Small Mammals
Ye-Hyun Kim, Katrina M. Schrode, and Amanda M. Lauer

17. Behavioral Models Loudness, Hyperacusis, and Sound Avoidance
Richard Salvi, Connor Mauche, Hannah Thorner, Guang-Di Chen, and Senthilvelan Manohar

Subject Index List...