Main description:

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab.
Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures.

Contents:

1. In Vitro Models in Developmental Toxicology

Louise M. Winn

2. Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments

Jason M. Hansen and Ted B. Piorczynski

3. Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology

Charles A. Easley

4. Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model

Mika Suga and Miho K. Furue

5. Micromass Methods for the Evaluation of Developmental Toxicants

Omar J. Mohammed and Margaret K. Pratten

6. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System

France-Helene Paradis, Han Yan, Chunwei Huang, and Barbara F. Hales

7. Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology

Barbara D. Abbott

8. Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology

Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, and Sakhila K. Banu

9. Hepatotoxicity in Zebrafish Larvae

Kathryn Bambino, Joshua Morrison, and Jaime Chu

10. Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model

Joslynn G. Affleck and Virginia K. Walker

11. The Chicken Embryo as a Model in Developmental Toxicology

Michael R. Stark and Micah M. Ross

12. The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity

Cecilia Berg

13. Mouse Whole Embryo Culture

Emily W.Y. Tung and Louise M. Winn

14. Rat Whole Embryo Culture

Craig Harris

15. Rabbit Whole Embryo Culture

Terence R.S. Ozolins

16. Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology

Brendon K. Warner, Jonathan K. Alder, and Arminda Suli

17. Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish

Jonathon T. Hill

18. Assessment of Histiotrophic Nutrition Using Fluorescent Probes

Craig Harris

19. Examining Gene Expression Patterns through Whole Mount In Situ Hybridization

Jeffery R. Barrow

20. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos

Robert M. Zucker and John M. Rogers

21. Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection

Danielle M. Drake, Aaron M. Shapiro, and Peter G. Wells

22. Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA

Shama Bhatia and Peter G. Wells

23. Western Analysis of Breast Cancer 1 Protein (BRCA1)

Danielle M. Drake and Peter G. Wells

24. Regulation and Control of AP-1 Binding Activity in Embryotoxicity

Terence R.S. Ozolins

25. Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR)

Shama Bhatia, Jason Matthews, and Peter G. Wells

26. The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity

Rebecca Maciver and Terence R.S. Ozolins

27. Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining

Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, and Michelle Graf