Main description:

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Contents:

1-Basic idea about nucleic acids for non-experts

2-General steps during isolation of DNA and RNA

3-Phenol-chloroform DNA isolation method

4-RNA isolation by the guanidinium-acid-phenol method

5-Spin column-based isolation of nucleic acid

6-Isolation of plasmid DNA by alkaline lysis

7-DNA Isolation by Hydrophilic Ionic Liquid Treatment

8-Lithium Chloride based isolation of DNA and RNA

9-Isolation of Bacteriophage DNA by PEG Method

10-DNA isolation by Chelex Method

11-DNA isolation by Lysozyme and Proteinase K

12-Salting-out method for blood samples

13-CTAB or SDS based isolation of plant's DNA

14-DNA extraction by spooling method

15-Magnetic bead-based nucleic acid isolation

16-Density gradient-based nucleic acid isolation

17-DNA extraction from agarose gel though paper-strips

18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS

19-GENE CLONING AND VECTORS

20-POLYMERASE CHAIN REACTION (PCR)

21-Southern and Northern Blotting

22-GENOME MAPPING

23-Applications of DNA Sequencing Technologies for Current Research