Main description:

Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Contents:

Reverse transcriptase Part I (discovery, preparation, general utilization)
1. MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more
2. Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution

Reverse transcriptase Part II (RNA structure mapping and determination)
3. RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays
4. tRNA Structure-seq in vivo and in droplets
5. Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq
6. Sequencing-based analysis of RNA structures in living cells with SHAPE-MaP

RNA polymerase (discovery, preparation, development/engineering and application)
7. Making RNA: Using T7RNA polymerase to produce high yields of RNA from DNA templates
8. A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled with Strengthening Promoter Binding by a Targeted Gap in the DNA
9. Isolation of E. coli RNA polymerase transcription elongation complexes by selective photoreversible solid-phase immobilization

RNA ligase
10. RNA ligation using vaccinia RNA ligase enzyme: high yield RNA coupling for RNA circularization, adapter ligation and other applications
11. Chemical crosslinking and ligation methods for in vivo analysis of RNA structures and interactions

Post-transcriptional modification enzymes
12. Genome-wide detection of dihydrouridine modifications in RNA
13. BID-seq: The quantitative base-resolution method for pseudouridines in mammalian mRNA.
14. Quantitative DAMM-seq maps multiple RNA methylations in tRNA and mitochondrial dsRNA
15. Discovering RNA modification enzymes by a comparative genomics approach
16. Functional analysis of tRNA modification enzymes using Mutational profiling
17. Fluorescent labelling of tRNA for interaction studies with RNA modification enzymes
18. Synthesis of long RNA with a site-specific modification by enzymatic splint ligation

Endo/Exo nucleases
19. Characterizing RNase L-mediated mRNA decay in single cells
20. Characterization of RNase J
21. DICER cleavage assays
22.
23. DROSHA cleavage assays