Main description:

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.

Contents:

1. Mechanisms of Drug-Induced Cholestasis

Eva Gijbels and Mathieu Vinken

2. LC-MS/MS Analysis of Bile Acids in In Vitro Samples

Debbie Dewaele, Pieter Annaert, and Eef Hoeben

3. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, and Jan G. Hengstler

4. Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, and Pieter Annaert

5. The C-DILI (TM) Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity

Jonathan P. Jackson and Kenneth R. Brouwer

6. In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

Stef De Lombaerde, Sara Neyt, Christian Vanhove, and Filip De Vos

7. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

Tania Sousa, Rui E. Castro, Ana Coutinho, Cecilia M.P. Rodrigues, Manuel Prieto, and Fabio Fernandes

8. The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis

Reza Heidari and Hossein Niknahad

9. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

Benjamin L. Woolbright and Hartmut Jaeschke

10. Necrosome Formation and Necroptosis in Experimental Cholestasis

Marta Bento Afonso and Cecilia Maria Pereira Rodrigues

11. Proteomics in Primary Biliary Cholangitis

You Li, Ruqui Tang, Patrick S.C. Leung, Eric Gershwin, and Xiong Ma

12. Transcriptomics Analysis of Cholestatic Compounds In Vitro

Celine Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, and Lysiane Richert

13. Systems Microscopy of Stress Response Pathways in Cholestasis Research

Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, and Bob van de Water

14. Mouse Models for Diseases in the Cholangiocyte Lineage

Noemi Van Hul, Urban Lendahl, and Emma R. Andersson

15. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

Sanne Van Campenhout, Hans Van Vlierberghe, and Lindsey Devisscher

16. 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research

Elisa Pose, Pau Sancho-Bru, and Mar Coll

17. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

Sujit K. Mohanty, Bryan Donnelly, Haley Temple, and Gregory M. Tiao

18. Zebrafish as a Model to Study Cholestatic Liver Diseases

Duc-Hung Pham and Chunyue Yin

19. Set-Up and Use of HepaRG Cells in Cholestasis Research

Christiane Guguen-Guillouzo and Andre Guillouzo

20. Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis

Sreenivasa C. Ramaiahgari and Stephen S. Ferguson

21. Establishment of Sandwich Cultures of Primary Human Hepatocytes

Eva Gijbels, Tamara Vanhaecke, and Mathieu Vinken

22. Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes

Neel Deferm, Lysiane Richert, Pieter Van Brantegem, Tom De Vocht, Bing Qi, Peter de Witte, Thomas Bouillon, and Pieter Annaert

23. Ex Vivo Model in Cholestasis Research

Ruby E.H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, and Peter Olinga

24. Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies

Carol J. Soroka, David N. Assis, and James L. Boyer

25. Use of Biliary Organoids in Cholestasis Research

Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, and Ludovic Vallier

26. In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis

Sankalp Jain and Gerhard F. Ecker