Main description:

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

Contents:

Part I: Gene Mutation Assays

1. In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay

Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi

2. Erythrocyte-Based Pig-a Gene Mutation Assay

Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger

3. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich

Part II: Assays for Chromosomal Abnormalities

4. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

Pasquale Mosesso and Serena Cinelli

5. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry

Antonella Testa, Valentina Palma, and Clarice Patrono

6. Chromosomal Aberration Test in Human Lymphocytes

Christian Johannes and Guenter Obe

7. In Vivo Micronucleus Assay in Mouse Bone Marrow

Abhishek Kumar Jain and Alok Kumar Pandey

8. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells

Claudia Bolognesi and Michael Fenech

9. Micronucleus Analysis by Flow Cytometry

Azeddine Elhajouji and Pascal Stadelmann

10. Hen's Egg Test for Micronucleus Induction (HET-MN)

Kerstin Reisinger, Eva Dony, Thorsten Wolf, and Katrin Maul

11. Comparative Genomic Hybridization (CGH) in Genotoxicology

Adi Baumgartner, Veronika Hartleb, and Jim D. Taylor

Part III: Tests for Primary DNA Damage

12. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan

13. The Comet Assay in Human Biomonitoring

Diana Anderson and Julian Laubenthal

14. The Comet Assay in Marine Animals

Patrizia Guidi, Brett P. Lyons, and Giada Frenzilli

15. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson

16. Detection of DNA Double Strand Breaks Using Pulse Field Gel Electrophoresis

Vyom Sharma and Vishwa Mohan

17. Detection of UV-Induced Thymine Dimers

Vipin Kumar Yadav, Poorwa Awasthi, and Amit Kumar

Part IV: Assays in Plants and Alternate Animal Models

18. Micronucleus Assay with Tetrad Cells of Tradescantia

Miroslav Misik, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, Katarina Misikova, and Siegfried Knasmueller

19. The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

Ricard Marcos and Erico R. Carmona

Part V: In Silico Testing for Genetic Toxicology

20. In Silico Approaches in Predictive Genetic Toxicology

Meetali Sinha, Alok Dhawan, and Ramkrishnan Parthasarathi