Main description:

This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Analytical and Bioinformatics Methods for Studying Glycosylation
1. Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from ProteinsIan Walsh, Sophie Zhao, Katherine Wongtrakul-Kish, Matthew Choo, Shi Jie Tay, Christopher H. Taron, Pauline M. Rudd, and Terry Nguyen-Khuong
2. GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data Matthew P. Campbell, Sophie Zhao, Jodie L. Abrahams, Terry Nguyen-Khuong, and Pauline M. Rudd
3. An Interactive View of Glycosylation Julien Mariethoz, Davide Alocci, Niclas G. Karlsson, Nicolle H Packer, and Frederique Lisacek
4. Characterization and Analysis of Food-Sourced CarbohydratesLeonie J. Kiely and Rita M. Hickey
5. Comparative Glycomics Analysis of Mass Spectrometry DataYusen Zhou and Sriram Neelamegham
Part II Synthetic Biology and Glycoengineering Methods for Controlling Glycosylation
6. Cell Free Remodeling of Glycosylation of AntibodiesLeticia Martins Mota, Venkata Tayi, and Michael Butler
7. Rapid Antibody Glycoengineering in CHO Cells via RNA Interference and CGE-LIF N-GlycomicsPavlos Kotidis, Masue Marbiah, Roberto Donini, Itzcoatl A. Gomez, Ioscani Jimenez del Val, Stuart M. Haslam, Karen M. Polizzi, and Cleo Kontoravdi
8. In Silico Analysis of Therapeutic Antibody Aggregation and the Influence of GlycosylationHyesoo Jeon, Jerrard M. Hayes, and K. H. Mok
9. Recent Advances Towards Engineering Glycoproteins using Modified Yeast Display PlatformsAnjali Shenoy and Adam W. Barb
Part III Systems Biology and Computational Methods for Deciphering Glycosylation Networks
10. Computational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangements of Biosynthetic EnzymesBen West, A. Jamie Wood, and Daniel Ungar
11. O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation NetworksAndrew G. McDonald and Gavin P. Davey
12. Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme ProbesMeenakshi Singh, Michael Watkinson, Eoin M. Scanlan, and Gavin J. Miller
Part IV Glycosylation Biomarkers and Clinical Datasets
13. N-Glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass SpectrometryRichard A. Gardner, Paulina A. Urbanowicz, and Daniel I. R. Spencer
14. Lectin Histochemistry for Tissues and Cells, and Dual Lectin and Antibody Co-LocalizationSiobhan S. McMahon and Michelle Kilcoyne
15. Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate CancerEsther Llop and Rosa Peracaula
16. Metabolic Labelling of Primary Neurons using Carbohydrate Click-ChemistryJerrard M. Hayes, Darren M. O'Hara, and Gavin P. Davey
17. Monitoring the Sialome on Human Immune CellsLaura K. O'Farrell, Alexander Fraser, and Gavin P. Davey
Subject Index List...