Main description:

This second edition volume expands on the previous edition with updates about the latest state-of-the-art techniques used in leading hemostasis and thrombosis laboratories for diagnosis and exclusion of hemorrhagic and thrombotic diseases. The chapters in this book are organized into seven parts. Part One provides a general overview on hemostasis and thrombosis, preanalytical issues in testing, and routine hemostasis assays. Part Two covers laboratory testing for thrombophilia, including reviews for activated protein C resistance, protein C, lupus anticoagulant testing, and antiphospholipid antibodies. Part Three addresses monitoring continuous anticoagulant infusions and measuring the effects of oral anti-thrombotic therapy. Part Four talks about heparin induced thrombocytopenia and vaccine induced immune thrombotic thrombocytopenia. Part Five and Six cover ADAMTS13 activity testing and new information on bleeding disorders such as chromogenic factor VIII assays, measurement of emicizumab, and treatment of hemophilia A and B. Finally, Part Seven discusses global assays, research applications, and postanalytical considerations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Hemostasis and Thrombosis: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers struggling to identify the appropriate methods for hemostasis and thrombosis testing, or who seek additional expert guidance on such testing.

Contents:

Dedication...Preface...Table of Contents...Contributing Authors...
Part I Introductory Chapters and Routine Coagulation Assays
1. Hemostasis and Thrombosis: An Overview Focusing on Associated Laboratory Testing to Diagnose and Help Manage Related DisordersEmmanuel J. Favaloro, Robert C. Gosselin, Leonardo Pasalic, and Giuseppe Lippi
2. Preanalytical Variables in Hemostasis TestingRobert C. Gosselin
3. Automation in the Thrombosis and Hemostasis LaboratoryEric Salazar and Russell Higgins
4. Ultracentrifugation for Coagulation TestingRobert C. Gosselin
5. Harmonization of Hemostasis Testing Across a Large Laboratory Network: An Example from AustraliaEmmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
6. Auto-Validation of Routine Coagulation/Hemostasis Assays with Reflex Testing of Abnormal Test ResultsEmmanuel J. Favaloro, Soma Mohammed, Violeta Ule Priebbenow, Ronny Vong, and Leonardo Pasalic
7. Laboratory Testing of Hemostasis in Pregnancy: A Brief OverviewMaha Othman and Anushka Pradhan
8. Protocols for D-Dimer Measurements for Aid to Diagnosis or Exclusion of Venous ThromboembolismRobert C. Gosselin
9. Performance and Interpretation of Clot Waveform AnalysisMaria Filomena Ruberto, Francesco Marongiu, and Doris Barcellona
Part II Thrombophilia Related Chapters
10. Laboratory Evaluation of ThrombophiliaRichard A. Marlar
11. Laboratory Testing for Activated Protein C Resistance (APCR): An UpdateEmmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
12. Evaluation of Activated Protein C Resistance using Thrombin Generation TestJonathan Douxfils, Celine Bouvy, and Laure Morimont
13. Performance of Chromogenic Protein C (PC) TestingRebecca Adams, Robyn Coleman, and Timothy Stanton
14. Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic VariantsTadej Pajic
15. An Overview of Laboratory Testing for Antiphospholipid AntibodiesEmmanuel J. Favaloro and Leonardo Pasalic
16. Lupus Anticoagulant Testing: Taipan Snake Venom Time with Ecarin Time as Confirmatory TestGary W. Moore
17. Lupus Anticoagulant Testing: Dilute Prothrombin Time (dPT)Gary W. Moore
18. An Acute Need Inspiration: Autoneutralization of Lupus Anticoagulants in Affected Prothrombin Times (PT) and Activated Partial Thromboplastin Times (APTT)Robert C. Gosselin
19. Antiphospholipid Antibody Testing for Anti-Cardiolipin and Anti- 2Glycoprotein I Antibodies using Chemiluminescence-Based PanelsEmmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
20. Laboratory Testing for Non-Criteria Antiphospholipid Antibodies-Antiphosphatidylserine/Prothrombin Antibodies (aPS/PT)Katrien M.J. Devreese
21. Laboratory Testing for Non-Criteria Antiphospholipid Antibodies: Antibodies Towards the Domain One of Beta2-Glycoprotein I (aDI)Katrien M.J. Devreese
Part III Measuring or Monitoring Anticoagulants and Antithrombobotic Therapy
22. An Overview of Heparin Monitoring with the Anti-Xa AssayChristina L. Dean
23. Ecarin Based Methods for Measuring Thrombin InhibitorsRobert C. Gosselin
24. Assays to Monitor BivalirudinSimon Davidson
25. Monitoring of Anti-Platelet TherapySimon Davidson
PART IV Heparin Induced Thrombocytopenia (HIT) and Vaccine Induced Immune Thrombotic Thrombocytopenia (VITT)
26. Heparin Induced Thrombotic Thrombocytopenia (HITT) and Vaccine Induced Immune Thrombotic Thrombocytopenia (VITT): Similar by DifferentEmmanuel J. Favaloro and Leonardo Pasalic
27. Functional Testing for Heparin Induced Thrombocytopenia using Whole Blood Multiplate Impedance AggregometryMarie-Christine Morel-Kopp
28. Multiple Electrode Aggregometry (Multiplate): Functional Assay for Vaccine Induced (Immune) Thrombotic Thrombocytopenia (VITT)Yvonne Kong, Geoffrey Kershaw, Yuet Kee Suki Fu, and Freda Passam
29. Whole Blood Procoagulant Platelet Flow Cytometry Protocol for Heparin-Induced Thrombocytopenia (HIT) and Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) TestingChristine S. M. Lee, Matthew C. Powell, and Vivien M. Chen
30. Serotonin Release Assay: Functional Assay for Heparin and Vaccine Induced (Immune) Thrombotic ThrombocytopeniaMayuko Kondo, Dea Donikian, and Timothy A. Brighton
PART V ADAMTS13
31. An Overview of Laboratory Testing for ADAMTS13Emmanuel J. Favaloro and Leonardo Pasalic
32. Automated and Rapid ADAMTS13 Testing using Chemiluminescence: Utility for Identification or Exclusion of TTP and BeyondEmmanuel J. Favaloro, Kent Chapman, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
33. Identification of ADAMTS13 Inhibitors in Acquired TTPEmmanuel J. Favaloro, Kent Chapman, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
34. ADAMTS13 Activity: Screening Test ProtocolGary W. Moore, Margaret Griffiths, and Nikolaus B. Binder
35. ADAMTS13 Activity Measurement by ELISA and Fluorescence Resonance Energy Transfer AssayGary W. Moore, Marcos Llusa, Margaret Griffiths, and Nikolaus B. Binder
36. ADAMTS13 Antibody and Inhibitor AssaysGary W. Moore, Helga Vetr, and Nikolaus B. Binder
PART VI Bleeding Disorders
37. A Review of Factor VIII and Factor IX Assay Methods for Monitoring Extended Half-Life Products in Haemophilia A and BSunil Abraham an Elizabeth Duncan
38. Measuring Emicizumab Levels in the Hemostasis LaboratoryGeoffrey Kershaw and Caroline Dix
39. Chromogenic Factor VIII Assay for Patients with Haemophilia A and On Emicizumab TherapyOlivia A. Yacoub and Elizabeth M. Duncan
40. Assessment of Platelet Function by Automated Light Transmission AggregometryMayukh K. Sarkar and Christine Hinz
41. Assessment of Platelet Function by High-Throughput Screening Light Transmittance Aggregometry: Optimul AssayMelissa V. Chan, Marie Lordkipanidze, and Timothy D. Warner
42. Preparing Surrogate Abnormal Quality Samples for Platelet Function Studies and Viscoelastic TestingRobert C. Gosselin
43. Laboratory Testing for von Willebrand Disease Using a Composite Rapid 3-Test Chemiluminescence-Based von Willebrand Factor Assay PanelEmmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
44. Laboratory Testing for von Wilebrand Factor Activity by a Glycoproein Ib Binding Assay (VWF:GPIbR): HemosIL von Willebrand Factor Ristocetin Cofactor Activity on ACL TOP (R)Omid Seidizadeh and Flora Peyvandi
45. Laboratory Testing for von Willebrand Factor:Factor VIII Binding for Diagnosis or Exclusion of Type 2N von Willebrand Disease: An UpdateEmmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic
46. Automation of a Factor XIII Activity Assay Utilizing a Plasma Blank MeasurementKristi J. Smock and Karen A. Moser
PART VII Global Assays, Research Applications, and Postanalytical Considerations
47. Measurement of Blood Viscoelasticity using ThromboelastographyOksana Volod and Alice Runge
48. The TEG 5000 (c) System: System Description and Protocol for MeasurementsOksana Volod and Alice Runge
49. The TEG 6s (c) System: System Description and Protocol for MeasurementsOksana Volod and Alice Runge
50. The Quantra (R) System: System Description and Protocols for MeasurementsOksana Volod and Francesco Viola
51. Modified Rotational Thromboelastometry Protocol using Tissue Plasminogen Activator for Detection of Hypofibrinolysis and HyperfibrinolysisJulie Brogaard Laren, Christine Lodberg Hvas, and Anne-Mette Hvas
52. Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness using Scanning Electron MicroscopyTengyi Cai, Conor McCafferty, Suelyn Van Den Helm, Natasha Letunica, Chantal Attard, Paul Monagle, and Vera Ignjatovic
53. Post-Analytical Issues in Hemostasis and Thrombosis Testing: An UpdateEmmanuel J. Favaloro, Robert C. Gosselin, Leonardo Pasalic, and Giuseppe Lippi
Subject Index List...