Main description:

This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing.

Contents:

Acknowledgement...Preface...Table of Contents...Contributing Authors...
Part I In Vitro Toxicological High Throughput Screening Methods
1. Cell-Based Assays to Identify ERR and ERR/PGC ModulatorsCaitlin Lynch, Jinghua Zhao, and Menghang Xia
2. Mitochondrial Membrane Potential AssaySrilatha Sakamuru, Jinghua Zhao, Matias S. Attene-Ramos, and Menghang Xia
3. Cell-Based hERG Inhibition Assay in a High-Throughput FormatJinghua Zhao, and Menghang Xia
4. Identifying CAR Modulators Utilizing a Reporter Gene AssayCaitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia
5. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 CellsYitong Liu
6. Acetylcholinesterase Inhibition Assays for High-Throughput ScreeningShuaizhang Li, Andrew J. Li, Michael F. Santillo, and Menghang Xia
7. Cell-Based Assays to Identify Modulators of Nrf2/ARE PathwayZhengxi Wei, Jinghua Zhao, Li Zhang, and Menghang Xia
Part II In Vitro Toxicological High Content Screening Methods
8. Cell-Based Imaging Assay for Detection of PhospholipdosisLi Zhang, Shuaizhang Li, and Menghang Xia
9. GFP-LC3 High-Content Assay for Screening Autophagy ModulatorsLi Zhang, Jinghua Zhao, Wen-Xing Ding, and Menghang Xia
Part III Three-Dimensional Cell System for Toxicological High Throughput Screening
10. Generation of iPSC-Derived Brain Organoids for Drug Testing and Toxicological EvaluationHa Nam Nguyen
Part IV In Vivo Toxicological High Throughput Screening Methods
11. Zebrafish Behavioral Assays in ToxicologySubham Dasgupta, Michael T. Simonich, and Robert L. Tanguay
Part V In Silico High Throughput Screening Toxicity Data Analysis
12. High Throughput Screening Assay Profiling for Large Chemical DatabasesDaniel P. Russo and Hao Zhu
13. A Quantitative High Throughput Screening Data Analysis Pipeline for Activity ProfilingRuili Huang
14. CurveP Method for Rendering High Throughput Screening Dose-Response Data into Digital FingerprintsAlexander Sedykh
15. Accounting for Artifacts in High Throughput Toxicity AssaysJui-Hua Hsieh
16. Automatic Quantitative Structure-Activity Relationship Modeling to Fill Data Gaps in High-Throughput ScreeningHeather L. Ciallella, Elena Chung, Daniel P. Russo, and Hao Zhu
17. Use In Silico and In Vitro Methods to Screen Hepatotoxic Chemicals and CYP450 Enzyme InhibitorsYitong Liu
Subject Index List...