Main description:

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy.

Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Contents:

1. Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes

Giuliano Mazzini and Marco Danova

Part I - Vital histochemistry

2. Autofluorescence Label-Free Imaging of Liver Reticular Structure

Anna C. Croce, Giuseppina Palladini, Andrea Ferrigno and Mariapia Vairetti

3. Lysosome Imaging Based on Fluorescent Carbon Dots

Shuo Guo, Yuanqiang Sun and Zhaohui Li

4. Oxidative and Nitrosative Stress Detection in Human Sperm using Fluorescent Probes

Sara Escada-Rebelo and Joao Ramalho-Santos

5. Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis

Patrizia Vaghi, Amanda Oldani, Paola Fulghieri, Lidia Pollara, Enza Maria Valente and Virginie Sottile

Part II - Carbohydrate histochemistry

6. Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions

Susan A. Brooks

7. Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans

David Sanchez-Porras, Juan Varas, Carlos Godoy-Guzman, Fabiola Bermejo-Casares, Sebastian San Martin and Victor Carriel

8. Combined Lectin- and Immuno-Histochemistry (CLIH) for Fluorescence Microscopy

Dasa Zupancic, Mateja Erdani Kreft and Rok Romih

Part III - Protein histochemistry

9. Immunofluorescence Labelling of Skeletal Muscle in Development, Regeneration, and Disease

Marie E. Esper, Kasun Kodippili and Michael A. Rudnicki

10. Immunohistochemical Detection of the Autophagy Markers LC3 and P62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissues

Sabina Berezowska and Jose A. Galvan

11. Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin Fixed and Paraffin Embedded Tissues.

Tereza Losmanova, Mario P. Tschan, Jose A. Galvan and Sabina Berezowska

12. Immunogold Labelling of Milk Proteins at Transmission Electron Microscopy

Paolo D'incecco

13. Rediscover Potassium Permanganate as a Stain for Basic Proteins on Ultrathin Sections at Transmission Electron Microscopy

Lorena Zannino, Claudio Casali and Marco Biggiogera

Part IV - Lipid histochemistry

14. Tissue Fixation and Processing for the Histological Identification of Lipids

David Sanchez-Porras, Fabiola Bermejo Casares, Ramon Carmona, Tamara Weiss, Fernando Campos and Victor Carriel

15. Staining Methods for Normal and Regenerative Myelin in the Nervous System

Oscar D. Garcia-Garcia, Tamara Weiss, Jesus Chato-Astrain, Stefania Raimondo and Victor Carriel

16. Nile Red and BODIPY Staining Of Lipid Droplets in Mouse Oocytes and Embryos

Simona Bisogno, Lukasz Gasior and Grazyna E. Ptak

Part V - Nuclear histochemistry

17. Chromatin Dispersion Test to Assess DNA Damage in Cervical Epithelial Cells

Elva I. Cortes-Gutierrez, Jose L. Fernandez, Martha I. Davila-Rodriguez, Carlos Garcia de la Vega and Jaime Gosalvez

18. Uranyl-free staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy

Maria Assunta Lacavalla and Barbara Cisterna

19. Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapours

Claudio Casali, Lorena Zannino, and Marco Biggiogera

Part VI - Plant histochemistry

20. Localising Molecules in Plant Cell Walls using Fluorescence Microscopy

Lloyd A. Donaldson

21. Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro

Oriane Morel, Corentin Spriet, Cedric Lion, Fabien Baldacci-Cresp, Garance Pontier, Marie Baucher, Christophe Biot, Simon Hawkins and Godfrey Neutelings

22. Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls

Youssef Chebli and Anja Geitmann

23. Staining Starch with Iodine Solution

Shengnan Zhao, Yinhui Ren and Cunxu Wei

24. Histochemical Analysis of Plant Secretory Structures

Diego Demarco

Part VII - Histochemistry for nanoscience

25. Alcian Blue Staining to Visualize Intracellular Hyaluronic-Acid-Based Nanoparticles

Mathieu Repellin, Flavia Carton, Giovanna Lollo and Manuela Malatesta

26. Prussian Blue Staining to Visualize Iron Oxide Nanoparticles

Valeria Bitonto, Francesca Garello, Arnaud Scherberich, and Miriam Filippi

27. Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy

Manuela Costanzo and Manuela Malatesta

28. Fluorescent Labelling of Lignin Nanocapsules with Fluorol Yellow 088

Franco Cheli, Sara Falsini, Maria Cristina Salvatici, Sandra Ristori, Emilio Corti, Irene Costantini, Cristina Gonnelli, Francesco Saverio Pavone and Alessio Papini