Main description:

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.

Contents:

1. Flow Cytometry Analysis to Identify Human CD8+ T-Cells

Jacqueline Flynn and Paul Gorry

2. Flow Cytometry Analysis to Identify Human CD4+ T-Cell Subsets

Jacqueline Flynn and Paul Gorry

3. Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T-Cells Redirected with Antigen-Specific Receptors

Toshiki Ochi, Masaki Maruta, and Naoto Hirano

4. In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells

Taisuke Kondo, Yuuki Imura, Makoto Ando, Shunsuke Chikuma, and Akihiko Yoshimura

5. Human iPSC Generation from Antigen-Specific T-Cells

Toshinobu Nishimura, Yukiko Murmann, and Hiromitsu Nakauchi

6. In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Shoichi Iriguchi and Shin Kaneko

7. Redifferentiation of Adaptive Naive-Like CTL from T-Cell-Derived iPSC

Yohei Kawai and Shin Kaneko

8. In Vitro Differentiation of T-Cell: From Human iPS Cells in Feeder Free Condition

Yutaka Yasui, Yasumichi Hitoshi, and Shin Kaneko

9. Differentiating CD8ab T-Cells from TCR Transduced iPSCs for Cancer Immunotherapy

Atsutaka Minagawa and Shin Kaneko

10. In Vitro Differentiation of T-Cell: From CAR-Modified T-iPSC

Tatsuki Ueda and Shin Kaneko

11. In Vitro Differentiation of T-Cells: From Non-Human Primate Induced Pluripotent Stem Cells

Shoichi Iriguchi and Shin Kaneko

12. An Improved Method to Produce Clinical Scale Natural Killer Cells from Human Pluripotent Stem Cells

Huang Zhu and Dan S. Kaufman

13. In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T-Cells

Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima, Hideki Ohdan, Shin Kaneko, and Yasushi Uemura

14. In Vitro Differentiation of T-Cells from Murine Pluripotent Stem Cells

Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song

15. Clonogenic Culture of Mouse Thymic Epithelial Cells

Miho Sekai, Jianwei Wang, and Yoko Hamazaki

16. Single-Cell Transcriptome Analysis of T-Cells

Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, and Fabio Luciani

17. Structural Modeling of Lymphocyte Receptors and Their Antigens

Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila, Zichang Xu, Kazutaka Katoh, and Daron M. Standley

18. Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers

Tinhinane Fali, Camille K'Ros, Victor Appay, and Delphine Sauce

19. Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

Hideyuki Oguro

20. Using the Inducible Caspase9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking

Jonathan Villanueva, Toshinobu Nishimura, and Hiromitsu Nakauchi