(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.
Contents:
1. Flow Cytometry Analysis to Identify Human CD8+ T-Cells
Jacqueline Flynn and Paul Gorry
2. Flow Cytometry Analysis to Identify Human CD4+ T-Cell Subsets
Jacqueline Flynn and Paul Gorry
3. Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T-Cells Redirected with Antigen-Specific Receptors
Toshiki Ochi, Masaki Maruta, and Naoto Hirano
4. In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells
Taisuke Kondo, Yuuki Imura, Makoto Ando, Shunsuke Chikuma, and Akihiko Yoshimura
5. Human iPSC Generation from Antigen-Specific T-Cells
Toshinobu Nishimura, Yukiko Murmann, and Hiromitsu Nakauchi
6. In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Shoichi Iriguchi and Shin Kaneko
7. Redifferentiation of Adaptive Naive-Like CTL from T-Cell-Derived iPSC
Yohei Kawai and Shin Kaneko
8. In Vitro Differentiation of T-Cell: From Human iPS Cells in Feeder Free Condition
Yutaka Yasui, Yasumichi Hitoshi, and Shin Kaneko
9. Differentiating CD8ab T-Cells from TCR Transduced iPSCs for Cancer Immunotherapy
Atsutaka Minagawa and Shin Kaneko
10. In Vitro Differentiation of T-Cell: From CAR-Modified T-iPSC
Tatsuki Ueda and Shin Kaneko
11. In Vitro Differentiation of T-Cells: From Non-Human Primate Induced Pluripotent Stem Cells
Shoichi Iriguchi and Shin Kaneko
12. An Improved Method to Produce Clinical Scale Natural Killer Cells from Human Pluripotent Stem Cells
Huang Zhu and Dan S. Kaufman
13. In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T-Cells
Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima, Hideki Ohdan, Shin Kaneko, and Yasushi Uemura
14. In Vitro Differentiation of T-Cells from Murine Pluripotent Stem Cells
Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song
15. Clonogenic Culture of Mouse Thymic Epithelial Cells
Miho Sekai, Jianwei Wang, and Yoko Hamazaki
16. Single-Cell Transcriptome Analysis of T-Cells
Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, and Fabio Luciani
17. Structural Modeling of Lymphocyte Receptors and Their Antigens
Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila, Zichang Xu, Kazutaka Katoh, and Daron M. Standley
18. Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers
Tinhinane Fali, Camille K'Ros, Victor Appay, and Delphine Sauce
19. Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells
Hideyuki Oguro
20. Using the Inducible Caspase9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking
Jonathan Villanueva, Toshinobu Nishimura, and Hiromitsu Nakauchi
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2020
Pages: 267
Weight: 537g
Availability: Available
Subcategories: General Issues