Main description:

This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for performing analytical and functional measurements in the kidney; techniques that are both topical and of widespread relevance to the study of kidney biology and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Third Edition aims to be comprehensive guide for researchers in the field.

Contents:

Part I: In vitro, in vivo and ex vivo models of kidney disease

1. Restoration of podocyte phenotype in culture

Eishin Yaoita

2. Protocols for the propagation, culture and characterization of kidney fibroblasts

Tim D. Hewitson and Edward R. Smith

3. Isolation of rat glomeruli and propagation of mesangial cells to study the kidney in health and disease

Tim D. Hewitson and Edward R. Smith

4. In vitro models to evaluate molecular permeability of the kidney filtration barrier

Dan Wang and Nicholas Ferrell

5. Generating human glomeruli from pluripotent stem cells for disease modelling and drug screening

Lorna J. Hale and Melissa H. Little

6. Large-scale production of kidney organoids from human pluripotent stem cells

Veronika Sander, Aneta Przepiorski, Neil A. Hukriede, and Alan J. Davidson.

7. Construction of a multitubular perfusable kidney-on-chip for the study of renal disease

Brice Lapin, Sarah Myram, Manh-Louis Nguyen, Giacomo Gropplero, Sylvie Coscoy, and Stephanie Descroix

8. A "kidney-on-the-chip" model composed of primary human tubular, endothelial, and white blood cells

Julian A. Marschner, Lucas Martin, Gregor Wilken, Maria Elena Melica, and Hans-Joachim Anders

9. Standardized protocol for the preparation of precision-cut kidney slices: a translational model of renal fibrosis

Michael Schou Jensen, Camilla Merrild, Rikke Norregaard, Peter Olinga, and Henricus A.M. Mutsaers

10. Culture of three-dimensional Madin-Darby Canine Kidney (MDCK) cysts for in vitro drug testing in polycystic kidney disease

Sayanthooran Saravanabavan and Gopala. K. Rangan

11. Zebrafish model to study podocyte function within the glomerular filtration barrier

Nina Sopel and Janina Muller-Deile

Part II: Imaging in kidney research

12. Magnetic Resonance Imaging (MRI) analysis of tissue sodium concentration in chronic kidney disease

Kylie Martin, Vijay Venkatraman, Angela Agostinelli, Braden Thai, Daniel Stab, Tim D. Hewitson, Sven-Jean Tan, Nigel D. Toussaint, and Patsy Robertson

13. Determination of interstitial collagen deposition and related topography using the second harmonic generation-based Histoindex platform

Sadman Bhuiyan, Robert E. Widdop, and Chrishan S. Samuel

14. Quantitative imaging of podocyte foot processes in the kidney using confocal and STED microscopy

David Unnersjoe-Jess

15. Synthesis and expression of a targeted, ferritin-based tracer for PET imaging of kidney glomeruli

Edwin J. Baldelomar, Kasey C. Emoto, Maria Veronica Clavijo Jordan, Jennifer R. Charlton, David E. Reichert, Neda Parvin, Courtnie Yokono, Bradley D. Hann, and Kevin M. Bennett

Part III: Analytical and functional measurements in the kidney

16. Multiplex in situ hybridization in the study of acute kidney injury

Agnieszka Masztalerz, Alex Gregorieff, Serge Lemay, and Tomoko Takano

17. Spatial transcriptomics in kidney tissue

Arti M. Raghubar, Joanna Crawford, Kahli Jones, Pui Y. Lam, Stacey B. Andersen, Nicholas A Matigian, Monica S.Y. Ng, Helen Healy, Andrew J. Kassianos, and Andrew J. Mallett

18. Assessment of kidney mitochondrial function by high-resolution respirometry, transmission electron microscopy and histological techniques

Marisol Orozco-Ibarra, Omar Emiliano Aparicio-Trejo, Alexis Paulina-Jimenez Uribe, Estefani Yaquelin Hernandez-Cruz, Ana Karina Aranda-Rivera, Isabel Amador- Martinez, Francisca Fernandez-Valverde, and Jose Pedraza-Chaverri

19. Transdermal measurement of glomerular filtration rate in preclinical research

Thomas P Mullins, Daniel Schock-Kusch, and Linda A Gallo

20. The BioHybrid Assay - a novel method for determining calcification propensity

Armand M.G. Jaminon, Asim C. Akbulut, Chris P. Reutelingsperger, and Leon J. Schurgers

21. Quantification of calciprotein particles (CPPs) in serum/plasma samples using a fluorescent bisphosphonate

Yutaka Miura, Hiroshi Kurosu, Makoto Kuro-o

22. Monitoring of Rho GTPase Activity in Podocytes

Jun Matsuda and Tomoko Takano