Main description:

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.

Contents:

Part I: Lung Innate, Immunity, and Inflammation

1. 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy

William McKleroy and Kenneth Lyn-Kew

2. Overview of Innate Lung Immunity and Inflammation

David W.H. Riches and Thomas R. Martin

Part II: Isolation and Characterization of Lung Innate Immune Cells

3. Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes

Sophie L. Gibbings and Claudia V. Jakubzick

4. Isolation and Characterization of Mouse Neutrophils

Niki D.J. Ubags and Benjamin T. Suratt

5. Isolation and Characterization of Mouse Fibroblasts

Benjamin L. Edelman and Elizabeth F. Redente

6. Isolation of Rat and Mouse Alveolar Type II Epithelial Cells

Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, and Rachel L. Zemans

7. Isolation and Characterization of Human Alveolar type II Cells

Beata Kosmider, Robert J. Mason, and Karim Bahmed

8. Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells

Di Jiang, Niccolette Schaefer, and Hong Wei Chu

9. Isolation and Characterization of Human Lung Myeloid Cells

Yen-Rei A. Yu and Robert M. Tighe

10. Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions

Xiaohui Fang and Michael A. Matthay

11. Measuring Innate Immune Function in Mouse Mononuclear Phagocytes

John Matthew Craig and Neil Raj Aggarwal

12. Measuring Neutrophil Bactericidal Activity

Kenneth C. Malcolm

13. Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches

Alexandra L McCubbrey and William J. Janssen

14. Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches

Adrianne L. Stefanski, Dorota S. Raclawska, and Christopher M. Evans

15. Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease

Nathan D. Jackson, Lando Ringel, and Max A. Seibold

16. Application of Proteomics in Lung Research

Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, and Richard Reisdorph

17. Application of Metabolomics in Lung Research

Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, and Richard Reisdorph

18. Functional Genomics in Murine Macrophages

Frank Fang-Yao Lee and Scott Alper

Part III: Analysis of Lung Innate Immunity and Inflammation

19. Assessment of Ozone-induced Lung Injury in Mice

Vandy P. Stober and Stavros Garantziotis

20. Lung Histological Methods

Aneta Gandjeva and Rubin M. Tuder

21. Intravital Microscopy in the Mouse Lung

Yimu Yang, Joseph A Hippensteel, and Eric P Schmidt

22. Mouse Models of Acute Lung Injury and ARDS

Franco R. D'Alessio

23. Mouse Models of Asthma

Magdalena M. Gorska

24. Animal Models of Pulmonary Fibrosis

David N. O'Dwyer and Bethany B. Moore

25. Mouse Models of COPD

Karina A. Serban and Irina Petrache

26. Mouse Models of COPD

Karina A. Serban and Irina Petrache

27. Delivery of Therapeutics to the Lung

Dominique N. Price and Pavan Muttil