Contents:

1. General

General Instructions

Laboratory Equipments and Procedures

Buffers and pH

Quality Control

2. Analysis of Urine and Cerebrospinal Fluid

Normal Urine: Characteristics and Analysis

Abnormal Constituents of Urine

Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography

Composition of Cerebrospinal Fluid

3. Quantitative Experiments

Principles of Colorimetry

Principles of Spectrophotometer

Estimation of Glucose

Glucose Tolerance Test

Estimation of Serum and Urine Creatinine: Creatinine Clearance

Estimation of Urea

Serum Proteins-Albumin: Globulin Ratio

Serum Total Cholesterol

Lipid Profile

Serum Calcium and Phosphorus

Serum Bilirubin

Serum Transaminases

Serum Alkaline Phosphatase

Serum Uric Acid

4. Equipments And Procedures

Point of Care Testing

pH Meter

Paper Chromatography and Thin Layer Chromatography

Protein Electrophoresis

Polyacrylamide Gel Electrophoresis

Electrolyte Analysis

Arterial Blood Gas Analyzer

Enzyme-Linked Immunosorbent Assay

Immunodiffusion

Autoanalyzer

DNA Isolation from Blood/Tissues

5. Function Tests

Gastric Function Tests and Analysis of Gastric Juice

Pancreatic Function Tests

Liver Function Tests

Analysis of Bile

Renal Function Tests

Thyroid Function Tests

Adrenal Function Tests

6. Food and Energy

Energy Content of Food and Glycemic Index

Fats in Food

7. Clinical Case Studies

Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions

8. Objective Structured Practical Examination

Objective Structured Practical Examination

9. Reagents

Reagent Preparation

10. Normal Values

Normal Values

Worksheet