Main description:

This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software, data analysis, new instrumentation, and new methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to be a useful practical guide to researchers to help further their study in this field.

Contents:

Part I. Innovations in Instrumental Methods for Mass Spectrometry Imaging

1. Gas-phase Ion/Ion Reactions for Lipid Identification in Biological Tissue Sections

Boone M. Prentice

2. MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging

Klaus Dreisewerd, Tanja Bien, and Jens Soltwisch

3. Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues

Jae Young Kim, Heejin Lim, and Dae Won Moon

4. Single-Cell Metabolomics with Rapid Determination of Chemical Formulas from Isotopic

Fine Structures

Laith Z. Samarah, Akos Vertes, and Christopher R. Anderton

5. TOF-SIMS Imaging of Biological Tissue Sections and Structural Determination using Tandem MS

Sebastiaan Van Nuffel and Alain Brunelle

Part II: New Types of Laser Absorption Methods in Mass Spectrometry Imaging

6. Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays

Laith Z. Samarah and Akos Vertes

7. Metal-assisted Laser Desorption Ionization Imaging Mass Spectrometry

Frederic Fournelle and Pierre Chaurand

8. Mass Spectrometry Imaging of Metabolites by Nanostructure Initiator Mass Spectrometry with Fluorinated Gold Nanoparticles

Amelia Palermo

9. Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Shu Taira and Katsuhiro Shiono

Part III. Quantitative Mass Spectrometry Imaging

10. Nanosecond Photochemical Reaction (nsPCR) for Enhanced Mass Spectrometric Identification, Quantification and Visualization of Metabolites and Neuropeptides

Gongyu Li, Yuan Liu, and Lingjun Li

11. Extraction and Up-Concentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging

Sanghwan Park and Chang Young Lee

12. The Development and Application of DropletProbe-Mass Spectrometry for Examining Biodistribution of Therapeutics

Bingming Chen, Marissa Vavrek, Mark T. Cancilla, and Vilmos Kertesz

13. Quantitative MS imaging with Liquid Microjunction Surface Sampling

Qian Wu and Hongmei Lu

Part IV: New Types of Applications

14. Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations

Kelly C. O'Neill, Maria Emilia Duenas, Evan Larson, Trevor T. Forsman, and Young Jin Lee

15. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen in Situ

Tara R. Hawkinson and Ramon C. Sun

16. MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration

Stepan Strnad, Veronika Strnadova, David Sykora, Josef Cvacka, Lenka Maletinska, and Vladimir Vrkoslav

17. Silver-doped Nano-DESI MSI for Increased Specificity and Sensitivity of Alkenes

Johan Lillja and Ingela Lanekoff

Part V: Innovations in Data Analysis

18. Extract Metabolomic Information from Mass Spectrometry Images using Advanced Data Analysis

Xiang Tian, Zhu Zou, and Zhibo Yang