Main description:

This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

Contents:

Preface...

Table of Contents...

Contributing Authors...

Part I Fundamentals

1. Metabolic Profiling: Topic, Challenges, Perspective

Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson

2. Quality Control and Validation Issues in LC-MS Metabolomics

Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson

3. Data Treatment for LC-MS Untargeted Analysis

Samantha Riccadonna and Pietro Franceschi

4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis

Michael Witting

Part II Methods

5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications

Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis

6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition

Filippos Michopoulos

7. LC-MS Untargeted Analysis

Elizabeth Want

8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts

Dimitra Benaki and Emmanuel Mikros

9. Untargeted GC-MS Metabolomics

atthaios- mmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa

10. Rat Fecal Metabolomics Based Analysis

Olga Deda, Helen G. Gika, and Georgios A. Theodoridis

11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol

Petr Husek, Zdenek Svagera, Dagmar Hanzlikova, Iva Opekarova, Lucie Rimnacova, Helena Zahradnickova, and Petr Simek

12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples

Rawi Ramautar

Part III Plant/Food and Other Applications

13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR

Jan Schripsema and Denise Dagnino

14. NMR Spectroscopy Protocols for Food Metabolomics Applications

Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros

15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study

Brian Farneti

16. LC-MS Untargeted Protocol for the Analysis of Wine

Panagiotis Arapitsas and Fulvio Mattivi

Part IV Life Science Applications

17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage

Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li

18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)

Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz

19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation

Aikaterina Siopi and Vassilis Mougios