Main description:

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I Differential Scanning Calorimetry
1. The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions
Marie-Lise Jobin and Isabel D. Alves

2. Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c
Shigeyoshi Nakamura and Shun-ichi Kidokoro

3. Value of DSC in Characterization and Optimization of Protein Stability
Katherine Bowers and Natalia Markova

4. Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC)
Philipp O. Tsvetkov and Francois Devred

Part II Isothermal Titration Calorimetry

5. Intrinsic Thermodynamics of Protein - Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design
Vaida Paketuryte, Asta Zubriene, John E. Ladbury, and Daumantas Matulis

6. Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions
Christopher P. Jones, Grzegorz Piszczek, and Adrian Ferre-D'Amare

7. ITC Studies of Ribosome/Antibiotics Interactions
Emma Schenckbecher, Benoit Meyer, and Eric Ennifar

8. High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry
Juan Ramirez and Yves Nomine

9. ITC Measurement for High Affinity Aptamers Binding to their Target Proteins
Ryo Amano, Tomohisa Furukawa, and Taiichi Sakamoto

10. Thermodynamics of Molecular Machines using Incremental ITC
Benoit Meyer, Cyrielle da Veiga, Philippe Dumas, and Eric Ennifar

11. Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry
Anna Solokhina, Gernot Bonkat, and Olivier Braissant

12. Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC)
Philipp O. Tsvetkov, Romain La Rocca, Soazig Malesinski, and Francois Devred

Part III Advanced Data Processing and Kinetics

13. Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC
Ibrahim Q. Saeed and Niklaas Buurma

14. Tinkering with Binding Polynomials in Isothermal Titration Calorimetry
Rafael Claveria-Gimeno, Sonia Vega, Olga Abian, and Adrian Velazquez-Campoy

15. Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine
Eva Munoz and Guillermo Franco

16. Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter
Eva Munoz, Juan Sabin, Javier Rial, Daniel Perez, Eric Ennifar, Philippe Dumas, and Angel Pineiro

17. Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry
Colette F. Quinn and Lee D. Hansen

18. Characterization of Enzymatic Reactions using ITC
Barbara Zambelli