Main description:

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Contents:

1. Detection of miRNAs

Afrah Bawazeer and David C. Prince

2. MicroRNA Identification, Target Prediction, and Validation for Crop Improvement

Vrantika Chaudhary, Sumit Jangra, Apurva Mishra, and Neelam R. Yadav

3. MicroRNA Detection with CRISPR/Cas

Xinyuan Qiu, Chuanyang Liu, Chushu Zhu, and Lingyun Zhu

4. Detection of MicroRNAs by Northern Blot

Claudia Martinho and Sara Lopez-Gomollon

5. MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore

Sotaro Takiguchi and Ryuji Kawano

6. Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor

Yuwen Zhao and Shue Wang

7. Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification

Yannick Rondelez and Guillaume Gines

8. Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters

Rocky Payet and Martina Billmeier

9. Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification

Sarah Ake, Swagatika Kamila, and Gangli Wang

10. Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine

Ludek Zavesky and Ondrej Slanar

11. Determining miRNA Expression Patterns in Xenopus

Marco Antonaci, Alice M. Godden, and Grant N. Wheeler

12. Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets

Ulf Schmitz

13. sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species

Cristina Gomez-Martin, Ernesto Aparicio-Puerta, and Michael Hackenberg

14. MicroRNA-Target Identification: A Combinatorial In Silico Approach

Taufiqul K.M. Arif, Rachel K. Okolicsanyi, Larisa M. Haupt, and Lyn R. Griffiths

15. An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis

Alice M. Godden, Marco Antonaci, and Grant N. Wheeler

16. Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering

Qianxin Wu, Yale S. Michaels, and Tudor A. Fulga