Main description:

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that's cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. The MicroRNA
Shao-Yao Ying, Donald C. Chang, and Shi-Lung Lin

2. Target mRNA Driven Biogenesis of Cognate MicroRNAs In Vitro
Mainak Bose and Suvendra N. Bhattacharyya

3. Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection
Anna Majer and Stephanie A. Booth

4. Isolation and Analysis of Exosomal Microsomal MicroRNAs from Ovarian Follicular Fluid
Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flavio Vieira Meireles, Quinton A. Winger, and Gerrit J. Bouma

5. Profiling of MicroRNAs in the Biofluids of Livestock Species
Jason Ioannidis, Judith Risse, and F. Xavier Donadeu

6. Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders
Gabriel Rodrigo Fries and Joao Quevedo

7. Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation
Max Hubner, Pedro AF Galante, Simone Kreth, and Ludwig Christian Hinske

8. How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and their Target/Pathway Prediction in Bovine Ovarian Cells
Anna E. Zielak-Steciwko and John A. Browne

9. Gene Silencing In Vitro and In Vivo using Intronic MicroRNAs
Shi-Lung Lin and Shao-Yao Ying

10. Mining Exosomal MicroRNAs from Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration
Sang-Ging Ong, Won Hee Lee, Zhou Yang, and Joseph C. Wu

11. Quantitative Analysis of Precursors MicroRNAs and their Respective Mature MicroRNAs in Cancer Exosomes Overtime
Nuno Bastos and Sonia A. Melo

12. Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets
Yoichi Robertus Fujii

13. In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
Jian Xu, Xuelian Xiao, and Daheng Yang

14. Isolation and Identification of Gene-Specific MicroRNAs
Shi-Lung Lin, Donald C. Chang, and Shao-Yao Ying

15. Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells
Masashi Fukuoka and Hirohiko Hohjoh

16. Screening miRNA for Functional Significance by 3D Cell Culture System
Bo Han

17. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs
Shi-Lung Lin and Shao-Yao Ying

18. Neonatal Rat Cardiomyocytes Isolation, Culture and Determination of MicroRNAs' Effects in Proliferation
Lichan Tao, Yihua Bei, Yongqin Li, and Junjie Xiao

19. Gene Manipulation with Micro RNAs at Single Human Cancer Cell
Andres Stucky, Xuelian Chen, and Jiang F. Zhong

20. Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis
Alyne Simoes, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro, and Xiaofeng Zhou

21. Transgene-Like Animal Models Using Intronic MicroRNAs
Shi-Lung Lin, Shin-Ju E. Chang, and Shao-Yao Ying

22. Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming
Zhonghui Zhang and Wen-Shu Wu

23. Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR302 Induction
Shi-Lung Lin and Shao-Yao Ying

24. The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms
Shao-Yao Ying, William Fang, and Shi-Lung Lin

25. Identification and Isolation of Novel Sugar-Like RNA Protecting Materials Glycylglycerins from Pluripotent Stem Cells
Shi-Lung Lin