Main description:

Mineralizing Vesicles: From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease presents the state-of-the art in the properties of mineralizing EVs and their potential clinical applications. Chapters present foundational biochemical and biological aspects of EVs, cover the role of EVs in bone ossification and in cardiovascular and cartilage-related diseases, focus on the enzymes catalyzing the inorganic phosphate and calcium turn-over and the dynamic properties of the vesicles' peripheral proteins, describe the role of inorganic phosphate and calcium ions and of autophagy on the biogenesis and function of mineralizing EVs, and more.

Over two decades of research in the book demonstrate the importance and promise of mineralizing EVs, with all aspects covered, including their composition, function in physiological and pathological processes, and the practical aspects of their research covered.

Contents:

1. General aspects of EVs in bone formation and remodeling
2. Physiological biomineralization. Part I: the role of matrix vesicles in skeletal calcification
3. Physiological biomineralization. Part II: the role of matrix vesicles in dental calcification
4. Physiological biomineralization. Part III: the role of intracellular vesicles and media vesicles
5. Pathological biomineralization. Part I: Mineralizing EVs in cardiovascular diseases
6. Pathological biomineralization. Part II: Mineralizing EVs in osteoarthritis
7. The biochemistry of mineralizing EVs. Part I: the role of phosphatases
8. The biochemistry of mineralizing EVs. Part II: the role of annexins and phosphate transporters
9. The role of calcium and phosphate in the release and function of mineralizing EVs
10. The role of autophagy in the release and function of mineralizing EVs
11. The role of mineralizing EVs in cell-cell communication
12. Working with mineralizing EVs. Part I: purification techniques
13. Working with mineralizing EVs. Part II: proteomic profiling
14. Working with mineralizing EVs: Part III: biophysical characterization
15. Working with mineralizing EVs: Part IV: biomimetic models of mineralizing extracellular vesicles
16. Working with mineralizing EVs. Part V: use of mineralizing extracellular vesicles in bone regeneration