Main description:

Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.

Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.

Contents:

1. Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells

2. Deformylation of nascent chains on the ribosome

3. Optimizing purification and activity assays of N-terminal methyltransferase complexes

4. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods

5. Biochemical and structural analysis of N-myristoyltransferase-mediated protein tagging

6. Kinetic and catalytic features of N-myristoyltransferases

7. Detecting N-terminal myristoylation using metabolic Alk12 labeling

8. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities

9. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms

10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification, interaction studies and enzymatic assays