Main description:

The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Part I: Overview

1 Morphological Survey from Neurons to Circuits of the Mouse Retina

Yoshihiko Tsukamoto

2 Measuring Retinal Function in the Mouse

Jan Kremers and Naoyuki Tanimoto

3 Modeling Retinal Diseases Using Genetic Approaches in Mice

Akiko Maeda and Tadao Maeda

Part II: Outer to Mid-Retina

4 Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells

Roni A. Hazim and David S. Williams

5 Two-photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo

Yoko Miura

6 RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models

Bhubanananda Sahu and Akiko Maeda

7 Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models

Minzhong Yu and Neal S. Peachey

8 Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic Peptides Targeting Dimer Interface

Sandeep Kumar, Alyssia Lambert, Jon Rainier, and Yingbin Fu

9 Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System in Retinal Rods of the Mouse

Clint L. Makino, Teresa Duda, Alexandre Pertzev, and Rameshwar K. Sharma

10 Microglia Analysis in Retinal Degeneration Mouse Models

Katharina Dannhausen, Khalid Rashid, and Thomas Langmann

11 Determination Of Mitochondrial Oxygen Consumption in the Retina Ex-vivo: Applications for Retinal Disease

Yogita K. Adlakha and Anand Swaroop

12 Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice

Arlene A. Hirano, Xue Liu, Nicholas C. Brecha, and Steven Barnes

13 Assessment of the Absolute Excitatory Level of the Retina by Flicker Erg

Naoyuki Tanimoto and Mathias W. Seeliger

14 Ex Vivo Functional Evaluation of Synaptic Transmission from Rods to Rod Bipolar Cells in Mice

Johan Pahlberg, Anurima Majumder, and Nikolai O. Artemyev

15 Functional And Morphological Analysis of Off Bipolar Cells

Chase B. Hellmer and Tomomi Ichinose

Part III: Mid- To Inner Retina

16 Immunohistochemical Phenotyping Of Mouse Amacrine Cell Subtypes

Denize Atan

17 Phenotyping of Gap-junctional Coupling in the Mouse Retina

Arndt Meyer, Shubhash C. Yadav, and Karin Dedek

18 Ganglion Cell Assessment in Rodents with Retinal Degeneration

Erica L. Fletcher, Ursula Greferath, Susmita Saha, Emily E. Anderson, and Kirstan A. Vessey

19 Morphological Identification of Melanopsin Expressing Retinal Ganglion Cell Subtypes in Mice

Seul Ki Lee and Tiffany M. Schmidt

20 Functional Assessment of Melanopsin-driven Light Responses in the Mouse: Multielectrode Array Recordings

Shi-Jun Weng, Jordan M. Renna, Wei-Yi Chen, and Xiong-Li Yang

21 In Vitro Assays for Mouse Muller Cell Phenotyping through Microrna Profiling in the Damaged Retina

Luis I. Reyes-Aguirre, Heberto Quintero, Brenda Estrada-Leyva, and Monica Lamas

22 Analysis Of Retinal Vascular Plexi and Interplexus Connections

Aaron B. Simmons and Peter G. Fuerst