Main description:

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1 Building a Two-Photon Microscope is Easy
Spencer LaVere Smith

2 Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas
Qiang Chen and Wei Wei

3 Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration
Alexandra Tran-Van-Minh, Nelson Rebola, Andreas Hoehne, and David A. DiGregorio

4 Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines
Ivar S. Stein, Travis C. Hill, Won Chan Oh, Laxmi K. Parajuli, and Karen Zito

5 Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System
Bart G. Borghuis

6 Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM
Paul R. Evans, Long Yan, and Ryohei Yasuda

7 Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling
Espen Hartveit and Margaret Lin Veruki

8 Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology
Espen Hartveit, Bas-Jan Zandt, and Margaret Lin Veruki

9 Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain
Denise M.O. Ramirez, Apoorva D. Ajay, Mark P. Goldberg, and Julian P. Meeks

10 Studying a Light Sensor with Light: Multiphoton Imaging in the Retina
Thomas Euler, Katrin Franke, and Tom Baden

11 Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach
Rune Enger, Rolf Sprengel, Erlend A. Nagelhus, and Wannan Tang

12 Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo
Lucy M. Palmer

13 Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy
Christopher J. Roome and Bernd Kuhn

14 High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals
Mate Marosi, Gergely Szalay, Gergely Katona, and Balazs Rozsa