Main description:

This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.

Contents:

Series Preface...Preface...Table of Contents...Contributing Authors...
1. Neuroproteomics: The MethodsKa Wan Li
2. Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen TissueSabine Spijker, Leonidas Faliagkas, and Priyanka Rao-Ruiz
3. Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density LatticeTatsuo Suzuki, Yoshinori Shirai, and Weidong Li
4. Laser Microdissection for Spatial Proteomics of Post Mortem Brain TissueDavid C. Hondius, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, and August B. Smit
5. High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun ProteomicsNing Chen, Mingwei Liu, Jun Qin, Wei Sun, and Fuchu He
6. Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)Ka Wan Li
7. Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag TipEiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
8. Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-ComplexesSophie J.F. van der Spek, August B. Smit, and Nikhil J. Pandya
9. Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass SpectrometryFan Liu
10. TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived NeuronsNikhil J. Pandya, David Avila, Tom Dunkley, Ravi Jagasia, and Manuel Tzouros
11. Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal CulturesMiguel A. Gonzalez-Lozano and Frank Koopmans
12. Quantitative Analysis of Mass Spectrometry-Based Proteomics DataThang V. Pham and Connie R. Jimenez
13. 2D-DIGE as a Tool in NeuroproteomicsFlorian Weiland
14. Neuropeptidomics of the Mammalian BrainFang Xie, Krishna D.B. Anapindi, Elena V. Romanova, and Jonathan V. Sweedler
15. MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and PeptidesJoerg Hanrieder, Henrik Zetterberg, and Kaj Blennow
16. A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric DisorderMartina Rosato, Titia Gebuis, Iryna Paliukhovich, Sven Stringer, Patrick F. Sullivan, August B. Smit, and Ronald E. van Kesteren