Main description:

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes NGS technological applications in the field of forensic DNA analysis. The book covers the shift from capillary electrophoresis (CE) to NGS platforms and the fundamentals of NGS technologies, applications and advancements. Sections provide an overview of NGS Technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis and data analysis software and the analysis of NGS data. Next, the book explores the useful applications of NGS based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.

With chapter contributions from an internationally array of experts and the inclusion of practical case studies, this book is a useful reference for academics and researchers in genetics, biotechnology, bioinformatics, biology and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply and validate NGS-based technologies.

Contents:

Part 1: Overview of NGS technology and forensic science 1. Transition of Capillary Electrophoresis to Next Generation Sequencing (NGS) for forensic DNA analysis: need of the hour 2. Overview of NGS platforms and technological advancements for forensic applications 3. Processing of biological samples for forensic NGS analysis 4. Sequence analysis and Data analysis software for forensic applications of NGS data 5. Sequence and statistical analyses of NGS data

Part 2: Useful applications of NGS based forensic DNA analysis 6. Applications of NGS in analysis of autosomal STRs 7. Applications of NGS in analysis of Y STRs 8. Applications of NGS in analysis of mitochondrial genome and discriminate heteroplasmy 9. Analysis of mutational events through NGS at high fidelity 10. Forensic relevance of SNP analysis by NGS 11. Ancestry studies by NGS technology 12. Drawing phenotypic inferences by NGS analysis 13. Applications of NGS for plant DNA typing 14. Applications of NGS for animal DNA typing 15. Forensic applications of epigenetic markers analysis through NGS 16. Forensic applications of NGS-based microRNA analysis 17. Forensic applications of NGS-based microbiome analysis 18. Applications of NGS in analysis of challenging samples 19. Detection of human body fluid through mRNA analysis using NGS 20. Utilization of NGS technology in population studies 21. Emerging uses of genetic genealogy in forensics

Part 3: Validations and Interpretation guidelines of NGS workflows 22. Troubleshooting and challenges of NGS technology in forensic use 23. Ethical issues of forensic application of NGS technology 24. Nomenclature and data management of forensic NGS data 25. Validation of NGS for casework at forensic DNA laboratories 26. Guidelines for NGS data analysis and interpretation of results for forensic use 27. Establishment of Databases for NGS based forensic outcomes