Main description:

This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I NO Treatment and Detection

1. A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems - Bell Pepper Fruits as a Model

Jose M. Palma, Carmelo Ruiz, and Francisco J. Corpas

2. Measurements of Intra-Oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode

Sana N. Khan, Roohi Jeelani, and Husam M. Abu-Soud

3. Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps

Emrah Eroglu, Helmut Bischof, Suphachai Charoensin, Markus Waldeck-Weiermaier, F. Wolfgang Graier, and Roland Malli

4. Detection of Nitric Oxide by Membrane Inlet Mass Spectrometry

John M. Goodwin, Carol C. Chrestensen, and Ellen W. Moomaw

5. Quantum Cascade Lasers-Based Detection of Nitric Oxide

Gracia Montilla-Bascon, Julien Mandon, Frans J.M. Harren, Luis AJ Mur, Simona M. Cristescu, and Elena Prats

6. Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Molluscs

Paula Mariela Gonzalez and Susana Puntarulo

Part II NO Modifications

7. Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques

Alicia Izquierdo-Alvarez, Daniel Tello, J. Daniel Cabrera-Garcia, and Anotonia Martinez-Ruiz

8. A Proteomics Workflow for Dual Labeling Biotin Switch Assay to Detect and Quantify Protein S-Nitroylation

Heaseung Sophia Chung, Christopher I. Murray, and Jennifer E. Van Eyk

9. Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins

Lili Zhang, Pengpeng Shang, Changbao Chen, Jie Zhou, and Shuhua Zhu

10. Measurement of S-Nitrosoglutathione in Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Dimitrios Tsikas and Erik Hanff

11. Analysis of Recombinant Protein S-Nitrosylation using the Biotin-Switch Technique

S. Aime, S. Hichami, D. Wendehenne, and O. Lamotte

12. Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathione Reductase as a Model Protein

Damian Guerra, Ian Truebridge, Stephen J. Eyles, Patrick Treffon, and Elizabeth Vierling

13. Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup followed by Liquid Chromatography-Electrospray Time-of-Flight Mass Spectrometry in Plants

Mounira Chaki, Beatriz Sanchez-Calvo, Alfonso Carreras, Raquel Valderrama, Juan C. Begara-Morales, Francisco J. Corpas, and Juan B. Barroso

14. Electrophoretic Detection and Confocal Microscopic Imaging by Tyrosine Nitrated Proteins in Plant Tissue

Dhara Arora, Neha Singh, and Satish C. Bhatla

15. Identification of NO-Sensitive Cysteine Residues using Cysteine Mutants of Recombinant Proteins

Azam Shekariesfahlan and Christian Lindermayr

16. Detection of S-Nitrosylated Nuclear Proteins in Pathogen-Treated Arabidopsis Cell Cultures using Biotin Switch Technique

Azam Shekariesfahlan and Christian Lindermayr17. Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols

Adil Hussain, Byung-Wook Yun, and Gary J. Loake

18. Nitro-Fatty Acid Detection in Plants by High-Pressure Liquid Chromatography Couples to Triple Quadrupole Mass Spectrometry

Capilla Mata-Perez, Maria N. Padilla, Beatriz Sanchez-Calvo, Juan C. Begara-Morales, Raquel Valderrama, Francisco J. Corpas, and Juan B. Barroso

19. Alche_Bioinformatic Prediction of S-Nitrosylation Sites in Large Protein Datasets

Rosario Carmona, M. Gonzalo Claros, and Juan de Dios Alche

Part III NO Detoxification

20. Biotin Switch Processing and Mass Spectrometry Analysis of S-Nitrosated Thioredoxin and its Transnitrosation Targets

Changgong Wu, Tong Liu, Yan Wang, Lin Yan, Chuanlong Cui, Annie Beuve, and Hong Li

21. Immunodetection of S-Nitrosoglutathione Reductase Protein in Plant Samples

Tereza Ticha, Lenka Luhova, and Marek Petrivalsky

22. Thioredoxin-Dependent Decomposition of Protein S-Nitrosothiols

Sophie Kneeshaw and Steven H. Spoel